Priming of human neutrophils is necessary for their activation by extracellular DNA
| Autor: | E. N. Popova, Maria V. Vitushkina, Ludmila A Zinovkina, Roman A. Zinovkin, Anastasia S. Prikhodko |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Mitochondrial DNA Neutrophils Priming (immunology) Biology GPI-Linked Proteins DNA Mitochondrial p38 Mitogen-Activated Protein Kinases Biochemistry Neutrophil Activation 03 medical and health sciences 0302 clinical medicine Antigens CD Extracellular Humans Phosphorylation Cells Cultured CD11b Antigen Granulocyte-Macrophage Colony-Stimulating Factor DNA General Medicine Neutrophil extracellular traps Molecular biology In vitro Nuclear DNA 030104 developmental biology Integrin alpha M 030220 oncology & carcinogenesis biology.protein Cell Adhesion Molecules |
| Zdroj: | Biochemistry (Moscow). 81:609-614 |
| ISSN: | 1608-3040 0006-2979 |
| DOI: | 10.1134/s0006297916060079 |
| Popis: | Extracellular plasma DNA is thought to act as a damage-associated molecular pattern causing activation of immune cells. However, purified preparations of mitochondrial and nuclear DNA were unable to induce neutrophil activation in vitro. Thus, we examined whether granulocyte-macrophage colony-stimulating factor (GM-CSF) acting as a neutrophil priming agent can promote the activation of neutrophils by different types of extracellular DNA. GM-CSF pretreatment greatly increased p38 MAPK phosphorylation and promoted CD11b/CD66b expression in human neutrophils treated with mitochondrial and, to a lesser extent, with nuclear DNA. Our experiments clearly indicate that GM-CSF-induced priming of human neutrophils is necessary for their subsequent activation by extracellular DNA. |
| Databáze: | OpenAIRE |
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