MIC-100, a new system for high-throughput phenotyping of instantaneous leaf photosynthetic rate in the field
Autor: | Shunsuke Adachi, Ayumu Imamura, Kazuki Taniyoshi, Ryo Mukai, Yu Tanaka, Kazuma Sakoda, Shun Sukemura |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Oryza sativa Field (physics) Coefficient of variation fungi Photosynthesis system Reproducibility of Results food and beverages Oryza Plant Science Biology Photosynthesis 01 natural sciences Photosynthetic capacity Carbon cycle Plant Leaves 03 medical and health sciences Horticulture 030104 developmental biology Soybeans Agronomy and Crop Science Throughput (business) 010606 plant biology & botany |
Zdroj: | Functional Plant Biology. 49:496-504 |
ISSN: | 1445-4416 1445-4408 |
DOI: | 10.1071/fp21029 |
Popis: | Photosynthesis occurs mainly in plant leaves and is a fundamental process in the global carbon cycle and in crop production. The exploitation of natural genetic variation in leaf photosynthetic capacity is a promising strategy to meet the increasing demand for crops. The present study reports the newly developed photosynthesis measurement system ‘MIC-100,’ with a higher throughput for measuring instantaneous photosynthetic rate in the field. MIC-100 is established based on the closed system and directly detects the CO2 absorption in the leaf chamber. The reproducibility, accuracy, and measurement throughput of MIC-100 were tested using soybean (Glycine max L. (Merr.)) and rice (Oryza sativa L.) grown under field conditions. In most cases, the coefficient of variance (CV) for repeated-measurements of the same leaf was less than 0.1. The photosynthetic rates measured with the MIC-100 model showed a significant correlation (R2 = 0.93–0.95) with rates measured by a widely used gas-exchange system. The measurement throughput of the MIC-100 is significantly greater than that of conventional open gas-exchange systems under field conditions. Although MIC-100 solely detects the instantaneous photosynthetic rate under a given environment, this study demonstrated that the MIC-100 enables the rough evaluation of leaf photosynthesis within the large-scale plant populations grown in the field. |
Databáze: | OpenAIRE |
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