Dimerizable Cationic Detergents with a Low cmc Condense Plasmid DNA into Nanometric Particles and Transfect Cells in Culture

Autor: Emmanuel Dauty, Thomas Blessing, Jean-Serge Remy, Jean-Paul Behr
Přispěvatelé: Conception et application de molécules bioactives (CAMB), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2001
Předmět:
Ornithine
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
Detergents
Population
Receptors
Cell Surface
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
02 engineering and technology
Gene delivery
Transfection
010402 general chemistry
01 natural sciences
Biochemistry
Micelle
Catalysis
Mice
chemistry.chemical_compound
Colloid and Surface Chemistry
Cations
Alkanes
Animals
Cysteine
Disulfides
Sulfhydryl Compounds
Particle Size
education
ComputingMilieux_MISCELLANEOUS
Micelles
Electrophoresis
Agar Gel
education.field_of_study
[CHIM.ORGA]Chemical Sciences/Organic chemistry
Cationic polymerization
3T3 Cells
General Chemistry
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
021001 nanoscience & nanotechnology
0104 chemical sciences
Kinetics
Electrophoresis
chemistry
Biophysics
Spermine
Particle size
0210 nano-technology
Dimerization
DNA
Plasmids
Zdroj: ResearcherID
Journal of the American Chemical Society
Journal of the American Chemical Society, American Chemical Society, 2001, 123 (38), pp.9227-9234. ⟨10.1021/ja015867r⟩
ISSN: 1520-5126
0002-7863
Popis: The size of condensed DNA particles is a key determinant for in vivo diffusion and gene delivery to cells. Gene molecules can be individually compacted by cationic thiol detergents into nanometric particles that are stabilized by oxidative conversion of the detergent into a gemini lipid. To reach the other goal, gene delivery, a series of cationic thiol detergents with various chain lengths (C(12)-C(16)) and headgroups (ornithine or spermine) was prepared, using a versatile polymer-supported synthetic strategy. Critical micelle concentrations and thiol oxidation rates of the detergents were measured. The formation and stability of complexes formed with plasmid DNA, as well as the size, xi-potential, morphology, and transfection efficiency of the particles were investigated. Using the tetradecane/ornithine detergent, a solution of 5.5 Kpb plasmid DNA molecules was converted into a homogeneous population of 35 nm particles. The same detergent, once oxidized, exhibited a typical lipid phase internal structure and was capable of effective cell transfection. The particle size did not increase with time. Surprisingly, the gel electrophoretic mobility of the DNA complexes was found to be higher than that of plasmid DNA itself. Favorable in vivo diffusion and intracellular trafficking properties may thus be expected for these complexes.
Databáze: OpenAIRE
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