Kinetics of Antibody Response in BALB/c and C57BL/6 Mice Bitten by Phlebotomus papatasi
| Autor: | Jitka Hostomska, Jesus G. Valenzuela, Michaela Vlkova, Lenka Zídková, Jan Drahota, Iva Rohousova, Petr Volf, Lucia Pohankova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Saliva
Time Factors Mouse Immunoglobulin G Mice 0302 clinical medicine Immune Response 0303 health sciences Mice Inbred BALB C biology medicine.diagnostic_test lcsh:Public aspects of medicine Animal Models 3. Good health Infectious Diseases Insect Proteins Female Antibody Research Article lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 030231 tropical medicine Immunology Enzyme-Linked Immunosorbent Assay BALB/c 03 medical and health sciences Model Organisms Western blot Antigen stomatognathic system parasitic diseases medicine Animals Salivary Proteins and Peptides Biology 030304 developmental biology Public Health Environmental and Occupational Health lcsh:RA1-1270 Immunoglobulin E biology.organism_classification Leishmania Virology Mice Inbred C57BL Phlebotomus Humoral immunity Antibody Formation biology.protein Parasitology Zoology |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases; Vol 6 PLoS Neglected Tropical Diseases, Vol 6, Iss 7, p e1719 (2012) |
| ISSN: | 1935-2735 1935-2727 |
| Popis: | Background Phlebotomine sand flies are blood-sucking insects transmitting Leishmania parasites. In bitten hosts, sand fly saliva elicits specific immune response and the humoral immunity was shown to reflect the intensity of sand fly exposure. Thus, anti-saliva antibodies were suggested as the potential risk marker of Leishmania transmission. In this study, we examined the long-term kinetics and persistence of anti-Phlebotomus papatasi saliva antibody response in BALB/c and C57BL/6 mice. We also tested the reactivity of mice sera with P. papatasi salivary antigens and with the recombinant proteins. Methodology/Principal Findings Sera of BALB/c and C57BL/6 mice experimentally bitten by Phlebotomus papatasi were tested by ELISA for the presence of anti-saliva IgE, IgG and its subclasses. We detected a significant increase of specific IgG and IgG1 in both mice strains and IgG2b in BALB/c mice that positively correlated with the number of blood-fed P. papatasi females. Using western blot and mass spectrometry we identified the major P. papatasi antigens as Yellow-related proteins, D7-related proteins, antigen 5-related proteins and SP-15-like proteins. We therefore tested the reactivity of mice sera with four P. papatasi recombinant proteins coding for most of these potential antigens (PpSP44, PpSP42, PpSP30, and PpSP28). Each mouse serum reacted with at least one of the recombinant protein tested, although none of the recombinant proteins were recognized by all sera. Conclusions Our data confirmed the concept of using anti-sand fly saliva antibodies as a marker of sand fly exposure in Phlebotomus papatasi–mice model. As screening of specific antibodies is limited by the availability of salivary gland homogenate, utilization of recombinant proteins in such studies would be beneficial. Our present work demonstrates the feasibility of this implementation. A combination of recombinant salivary proteins is recommended for evaluation of intensity of sand fly exposure in endemic areas and for estimation of risk of Leishmania transmission. Author Summary Leishmania major is the causative agent of zoonotic cutaneous leishmaniasis and Phlebotomus papatasi serve as the major vector. In endemic foci, rodents are the natural reservoirs of this disease. Thus, we studied anti-P. papatasi saliva antibody response in BALB/c and C57BL/6 mice that are commonly used as model organisms sensitive and resistant to cutaneous leishmaniasis, respectively. We followed the kinetics and persistence of specific antibody response in both mice strains and we characterized the main P. papatasi salivary antigens. We demonstrated that sand fly bites elicit production of specific IgG that reflect the intensity of sand fly exposure. In endemic areas, this could provide useful information about the effectiveness of anti-vector control programs. We also examined the reaction of mice sera with four P. papatasi recombinant proteins. Our data indicate that a combination of these proteins could be used instead of crude salivary gland homogenate for the monitoring of anti-sand fly saliva antibodies in natural hosts in endemic foci. |
| Databáze: | OpenAIRE |
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