Bacillus endozanthoxylicus sp. nov., an endophytic bacterium isolated from Zanthoxylum bungeanum Maxim leaves
| Autor: | Cheng-Gang Yang, Jia-Qin Xi, Yong-Hong Cao, Qi-Li Mi, Ming-Liang Zhu, Xue-Mei Li, Xiao-Yan Wang, Ming-He Mo, Li Ma, Shuai-Chao Zheng, Ling-Ling Yang |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial Zanthoxylum 0301 basic medicine China Bacillus Peptidoglycan Biology Diaminopimelic Acid Microbiology 03 medical and health sciences chemistry.chemical_compound Cell Wall RNA Ribosomal 16S Botany Phospholipids Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics Bacillus (shape) Base Composition Strain (chemistry) Fatty Acids fungi Nucleic Acid Hybridization Phosphatidylglycerols Vitamin K 2 Sequence Analysis DNA General Medicine 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques Spore Plant Leaves 030104 developmental biology chemistry Bacillus circulans Diaminopimelic acid Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 67:3699-3705 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | A Gram-stain-positive, rod-shaped, motile bacterium, designated as 1404T, was isolated from leaves of Chinese red pepper (Huajiao) (Zanthoxylum bungeanum Maxim) collected from Gansu, north-west China. Spores were not observed under a range of conditions. Strain 1404T was observed to grow at 15–45 °C and pH 6.0–10.0 and in presence of 0–5 % (w/v) NaCl concentration. The cell wall of strain 1404T was found to contain meso-diaminopimelic acid, and the predominant respiratory quinone was identified as MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as well as three unidentified polar lipids. The major fatty acids profile of strain 1404T consisted of iso-C15 : 0 (25.6 %), anteiso-C15 : 0 (18.4 %) and iso-C14 : 0 (12.1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1404T was affiliated to the genus Bacillus and was closely related to Bacillus oryzisoli 1DS3-10T, Bacillus benzoevorans DSM 5391T and Bacillus circulans DSM 11T with sequence similarity of 98.3, 98.2 and 96.9 %, respectively. The G+C content of the genomic DNA was determined to be 39.4 mol%. DNA–DNA hybridization values indicated that relatedness between strain 1404T and the type strains of closely related species of the genus Bacillus was below 41 %. Therefore, on the basis of the data from the polyphasic taxonomic study presented, strain 1404T represents a novel species of the genus Bacillus , for which the name proposed is Bacillus endozanthoxylicus sp. nov. The type strain is 1404T (=CCTCC AB 2017021T=KCTC 33827T). |
| Databáze: | OpenAIRE |
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