The Spectral-Domain Optical Coherence Tomography Findings Associated with the Morphological and Electrophysiological Changes in a Rat Model of Retinal Degeneration, Rhodopsin S334ter-4 Rats

Autor: Yuting Xie, Sei-ichi Ishiguro, Natsuki Monai, Reiko Tanabu, Kodai Yamauchi, Takayuki Gonome, Shizuka Takahashi, Mitsuru Nakazawa
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Retinal degeneration
medicine.medical_specialty
Rhodopsin
Article Subject
genetic structures
lcsh:Medicine
Interphotoreceptor matrix
General Biochemistry
Genetics and Molecular Biology
Retina
Rats
Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Optical coherence tomography
Ophthalmology
medicine
Electroretinography
Animals
General Immunology and Microbiology
medicine.diagnostic_test
biology
lcsh:R
Retinal Degeneration
Retinal
General Medicine
medicine.disease
eye diseases
Rats
Electrophysiology
Disease Models
Animal
030104 developmental biology
chemistry
biology.protein
Retinal Cone Photoreceptor Cells
sense organs
Rats
Transgenic
Erg
030217 neurology & neurosurgery
Tomography
Optical Coherence
Research Article
Photoreceptor Cells
Vertebrate
Zdroj: BioMed Research International
BioMed Research International, Vol 2018 (2018)
ISSN: 2314-6141
2314-6133
Popis: Purpose. To characterize the spectral-domain optical coherence tomography (SD-OCT) findings of the rhodopsin S334ter transgenic rats (line 4) in relation to the morphologic and electroretinographic features. Materials and Methods. Rhodopsin S334ter transgenic rats (line 4) were employed as a model of retinal degeneration. The Sprague-Dawley (SD) rats were used as a wild-type control. SD-OCT (Micron IV®; Phoenix Research Labs, Pleasanton, CA, USA) was performed on the S334ter rats (line 4) from postnatal days (P) 13−110. The longitudinal changes of the SD-OCT images were analyzed both qualitatively and quantitatively in comparison to those of SD rats. The SD-OCT images were also compared to the histological and electron microscopic findings from examination performed on P 22, 36, and 61. Full field combined rod and cone electroretinography (ERG) was performed and the relationship between the thickness of the retinal sublayers and the amplitudes of the a- and b-waves was further analyzed. Results. The photoreceptor inner and outer segment layer became diffusely hyperreflective in the SD-OCT images of the S334ter rats; these findings were not observed in the SD rats. This hyperreflective change corresponded to the degenerated inner and outer segments and the accumulation of the extracellular vesicles in the interphotoreceptor matrix. Quantitatively, the retinal outer sublayer and the photoreceptor sublayer in the S334ter rats became progressively thinner in comparison to those in the SD rats; the difference was statistically significant. The amplitudes of both the a- and b-waves on ERG were severely deteriorated in the S334ter rats. Discussion. The SD-OCT images in the S334ter rats noninvasively provided information regarding the pathological changes in the photoreceptors and the longitudinal changes of both qualitative and quantitative changes during retinal degeneration in the S334ter rats (line 4). The pathological features of the photoreceptor inner and outer segments can be detected on SD-OCT as diffuse hyperreflective changes in the photoreceptor layer.
Databáze: OpenAIRE
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