Iron overload inhibits calcification and differentiation of ATDC5 cells
| Autor: | Hiroyuki Nishimura, Tomoya Ohno, Hiromi Hagiwara, Nobuaki Hashimoto, Kenichi Mitsui |
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| Rok vydání: | 2011 |
| Předmět: |
Time Factors
Iron Blotting Western chemistry.chemical_element Gene Expression Siderophores Cartilage metabolism Matrix metalloproteinase Calcium Deferoxamine Biochemistry Ferric Compounds Chondrocyte Mice Chondrocytes Cell Line Tumor Matrix Metalloproteinase 13 medicine Animals Molecular Biology Collagen Type II Calcium metabolism biology Dose-Response Relationship Drug Chemistry Reverse Transcriptase Polymerase Chain Reaction Spectrometry X-Ray Emission Cell Differentiation General Medicine Cell biology Ferritin Quaternary Ammonium Compounds medicine.anatomical_structure Proteoglycan Ferritins biology.protein Microscopy Electron Scanning Ferric Proteoglycans medicine.drug Collagen Type X |
| Zdroj: | Journal of biochemistry. 151(1) |
| ISSN: | 1756-2651 |
| Popis: | There is a little information about the effects of iron overload on cartilage metabolism. In the present study, we examined the effects of excess iron on the differentiation and mineralization of cultured chondrocytes, ATDC5 cells. We used ferric ammonium citrate (FAC) as a ferric ion donor and desferrioxamine (DFO) as a ferric ion chelator. Neither chemical affected the production of proteoglycan, a marker of an early stage of ATDC5 differentiation. In contrast, FAC inhibited the deposition of calcium, a late-stage event in chondrocyte differentiation, by ATDC5 cells in a dose-dependent manner, and DFO accelerated it. Energy dispersive X-ray spectroscopy/scanning electron microscope analysis revealed that the levels of iron and calcium in cells treated with FAC were increased and decreased, respectively. Furthermore, FAC inhibited the expression of matrix metalloproteinase 13 mRNA, another marker of late-stage chondrocyte differentiation. In addition, we found that the heavy and light chains of ferritin were expressed specifically at a late stage of ATDC5 differentiation, and the levels of both proteins were enhanced by the addition of iron. These results suggest that iron overload might give rise to osteopenia and arthritis by inhibiting chondrocyte differentiation and mineralization. |
| Databáze: | OpenAIRE |
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