Application of enzymehistochemical methods to isolated subcellular fractions and to sucrose-ficoll density gradients
| Autor: | T. J. Haarsma, W. A. R. Huijbers, Mj Hardonk, J Koudstaal, F. W. J. Dijkhuis |
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| Rok vydání: | 1977 |
| Předmět: |
Male
Sucrose Histology Acid Phosphatase Ficoll Biology Cell Fractionation Leucyl Aminopeptidase chemistry.chemical_compound Nucleotidases Acetylglucosaminidase Centrifugation Density Gradient medicine Animals Molecular Biology Glucuronidase NADH Tetrazolium Reductase chemistry.chemical_classification Histocytochemistry Kupffer cell Cell Biology General Medicine Alkaline Phosphatase Subcellular localization Staining Microscopy Electron Medical Laboratory Technology Ducks medicine.anatomical_structure Enzyme Liver chemistry Biochemistry Hepatocyte Glucose-6-Phosphatase Microsome Anatomy General Agricultural and Biological Sciences Subcellular Fractions |
| Zdroj: | Histochemistry. 53:165-181 |
| ISSN: | 1432-119X 0301-5564 |
| DOI: | 10.1007/bf00498491 |
| Popis: | To compare histochemical and biochemical determinations of enzyme activities, enzymehistochemical procedures are applied to sections of pellets of subcelluar fractions. These investigations are of value to determine the subcellular localization of histochemically demonstrable enzyme activities and to test the homogeneity of an isolated fraction. In homogenating duckling liver a great part of the endothelial cells is not destructed and consequently is found in the nuclear fraction. Kupffer cell lysosomes land in the heavy mitochondrial fraction, whereas hepatocyte lysosomes are chiefly found in the light mitochondrial fraction. beta-Glucuronidase activity shows a preferentially microsomal localization. Application of enzymehistochemical staining reactions to discontinuous gradients and comparison with biochemical data provides additional information about the validity of an enzymehistochemical reaction. In rat liver the tetrazolium reductases show a distinctly dual localization: activity in the mitochondrial band and in microsomal bands. As to their localization in different bands of the gradients non-specific esterases demonstrate a clear pH-dependency. |
| Databáze: | OpenAIRE |
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