Population of Rh123dim human keratinocytes form holoclones
| Autor: | Jacek Puchala, Agnieszka Kwarciak, Justyna Drukala, Mariusz Z. Ratajczak, Marcin Majka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
keratinocytes
education.field_of_study General Immunology and Microbiology Epidermis (botany) QH301-705.5 General Neuroscience Population β1 integrin β1-integrin Cell sorting Biology General Biochemistry Genetics and Molecular Biology Human genetics Cell biology holoclones rhodamin123 epidermis Progenitor cell Biology (General) General Agricultural and Biological Sciences education |
| Zdroj: | Open Life Sciences, Vol 4, Iss 2, Pp 154-162 (2009) |
| ISSN: | 2391-5412 |
| Popis: | The aim of our studies was to develop an efficient strategy to isolate human early epidermal progenitors for experimental and potential clinical purposes. We employed fluorescence-activated cell sorting (FACS) to isolate cells that poorly accumulate metabolic Rhodamin123 (Rh123) dye. We noticed that similarly to a population of β1-integrin bright (β1bright) cells, a population of Rh123 dull (Rh123dim) cells is highly enriched for cells growing holoclones, colonies composed of the most primitive keratinocytes. Furthermore, Rh123dim cells express several morphological features of primitive undifferentiated cells and are also highly motile. We postulate that these cells could become an important source of epidermal progenitors to expand keratinocytes for clinical purposes. |
| Databáze: | OpenAIRE |
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