Quantification of the neurons of myenteric plexus of the bat molossus rufus
| Autor: | Nathalia G.E. Legnani, Edson Gerônimo, Luciana Kazue Otutumi, Ricardo de Melo Germano, Paula Montanhini Favetta, Lisiane de Almeida Martins, José Matheus Beltrami, Grazielli de Fátima Serenini |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Veterinary medicine Population bats Biology Giemsa stain Jejunum 03 medical and health sciences Molossus rufus 0302 clinical medicine enteric nervous system SF600-1100 morphology medicine education Myenteric plexus Neurons education.field_of_study General Veterinary Anatomy digestive tube Small intestine 030104 developmental biology medicine.anatomical_structure NADPH-diaphorase 030220 oncology & carcinogenesis Enteric nervous system Molossidae Neuron Nitrergic Neuron myenteric plexus |
| Zdroj: | Pesquisa Veterinária Brasileira v.40 n.6 2020 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
| Popis: | There are no studies that characterize the enteric nervous system (ENS) bats. The organization and density of myenteric neurons may vary according to the animal species, as well as the segment of the digestive tube considered. The nitric oxide is one of the key neurotransmitters present in the myenteric neurons, acting as a mediator in the smooth muscle relaxation. These neurons are evidenced by immunohistochemistry of nitric oxide synthase (NOS) or by NADPH-diaphorase histochemistry. In this sense, this study aimed to characterize the total neuronal population and subpopulation NADPH-d+ of the myenteric plexus present in the jejunum of the insectivore species Molossus rufus quantitatively. Five specimens were collected of M. rufus in a buffer area of the “Reserva Biológica das Perobas” in the microregion of Cianorte/PR. After the euthanasia, in a chamber saturated with isoflurane, segments were collected from the small intestine corresponding to the jejunum intended for two techniques for neuronal marking, Giemsa and NADPH-diaphorase, and a fragment to the histological technique of hematoxylin-eosin and Masson’s trichrome. All the procedures were approved by the “Comitê de Ética no Uso de Animais Unipar” (CEUA - protocol No. 34347/2017) and the “Instituto Chico Mendes de Conservação da Biodiversidade” (ICMBio - protocol No. 60061-1) The histological sections allowed to highlight the location of the myenteric plexus between the longitudinal and circular layers of the muscular tunic. The myenteric plexus had an average of total neuronal population (neurons Giemsa+) of 279.23 neurons/mm2, being the nitrergic neurons (neurons NADPH-d+) represented 20.4% of this total population, with an average of 58.14 neuron/mm2. Therefore, the collected data are consistent with previous studies in other mammalian species concerning the location of the myenteric plexus, as well as the neural myenteric proportion NADPH-d+ compared with the population of neurons Giemsa+. The gaps in the knowledge of ENS of bats limits comparative intraspecific and interspecific studies. |
| Databáze: | OpenAIRE |
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