An Escherichia coli system for evolving improved light-controlled DNA-binding proteins
| Autor: | G. Andrew Woolley, Carrie-Lynn Keiski, Katja M. Arndt, Stefan A. Hoffmann, Yih Yang Chen, Katherine E. Brechun, David R. McMillen, Yongjoo B. Kim, Mostafizur Mazumder |
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| Rok vydání: | 2015 |
| Předmět: |
Fluorescent reporter
Light Transcription Genetic Bioengineering Protein engineering Computational biology Biology Directed evolution medicine.disease_cause Bioinformatics Protein Engineering Biochemistry DNA-binding protein DNA-Binding Proteins Transcription (biology) medicine Escherichia coli Molecular Biology Psychological repression Biotechnology |
| Zdroj: | Protein engineering, designselection : PEDS. 28(9) |
| ISSN: | 1741-0134 |
| Popis: | Light-switchable proteins offer numerous opportunities as tools for manipulating biological systems with exceptional degrees of spatiotemporal control. Most designed light-switchable proteins currently in use have not been optimised using the randomisation and selection/screening approaches that are widely used in other areas of protein engineering. Here we report an approach for screening light-switchable DNA-binding proteins that relies on light-dependent repression of the transcription of a fluorescent reporter. We demonstrate that the method can be used to recover a known light-switchable DNA-binding protein from a random library. |
| Databáze: | OpenAIRE |
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