Nucleosome eviction in mitosis assists condensin loading and chromosome condensation
| Autor: | Xavier Robellet, Pascal Bernard, Esther Toselli-Mollereau, Christian H. Haering, Emilie Chautard, Lydia Fauque, Carlo Klein, Léo Mouillard, Lia N'Guyen, Sébastien Lemaire, Pénélope Legros, Clémence Hocquet, Christoph Schiklenk, Didier Auboeuf |
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| Přispěvatelé: | Laboratoire de biologie et modélisation de la cellule (LBMC UMR 5239), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
[SDV]Life Sciences [q-bio] Condensin Mitosis macromolecular substances General Biochemistry Genetics and Molecular Biology Mitotic chromosome condensation 03 medical and health sciences 0302 clinical medicine Acetyltransferases Schizosaccharomyces Nucleosome Molecular Biology Transcription factor ComputingMilieux_MISCELLANEOUS Genetics Adenosine Triphosphatases Base Composition General Immunology and Microbiology biology Cohesin General Neuroscience Histone acetyltransferase Articles Cell biology Nucleosomes DNA-Binding Proteins 030104 developmental biology Premature chromosome condensation Multiprotein Complexes biology.protein Schizosaccharomyces pombe Proteins Chromosomes Fungal 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | EMBO Journal EMBO Journal, EMBO Press, 2016, 35 (14), pp.1565-1581. ⟨10.15252/embj.201592849⟩ EMBO Journal, 2016, 35 (14), pp.1565-1581. ⟨10.15252/embj.201592849⟩ |
| ISSN: | 0261-4189 1460-2075 |
| Popis: | Condensins associate with DNA and shape mitotic chromosomes. Condensins are enriched nearby highly expressed genes during mitosis, but how this binding is achieved and what features associated with transcription attract condensins remain unclear. Here, we report that condensin accumulates at or in the immediate vicinity of nucleosome-depleted regions during fission yeast mitosis. Two transcriptional coactivators, the Gcn5 histone acetyltransferase and the RSC chromatin-remodelling complex, bind to promoters adjoining condensin-binding sites and locally evict nucleosomes to facilitate condensin binding and allow efficient mitotic chromosome condensation. The function of Gcn5 is closely linked to condensin positioning, since neither the localization of topoisomerase II nor that of the cohesin loader Mis4 is altered in gcn5 mutant cells. We propose that nucleosomes act as a barrier for the initial binding of condensin and that nucleosome-depleted regions formed at highly expressed genes by transcriptional coactivators constitute access points into chromosomes where condensin binds free genomic DNA. |
| Databáze: | OpenAIRE |
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