IV. Molecular biology of S-layers
| Autor: | Holger Scholz, Michèle Mock, Werner Lubitz, Stephan Howorka, Thaddée Gulik-Krzywicki, Hein J. Boot, José Berenguer, Peter H. Pouwels, Beatrix Kuen, Alberto J.L. Macario, Sonja Lechleitner, Carin P. A. M. Kolen, Stéphane Mesnage, Luis Angel Fernández-Herrero, Airi Palva, Gerhard Schroll, Nicolas Bayan, Martin J. Blaser, Margit Sára, Agnès Fouet, Gérard Leblon, Mohamed Chami, Stephanie Resch, Evelyne Tosi-Couture, G Olabarría, Michaela Truppe, Hubert Bahl, Everly Conway de Macario, Pierre Gounon, Emanuel Shechter |
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| Rok vydání: | 1997 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Bacillus ATP-binding cassette transporter Corynebacterium Microbiology Homology (biology) Corynebacterium glutamicum Cell Wall Amino Acid Sequence Gene Peptide sequence Genetics Antigens Bacterial Bacteria Base Sequence biology Thermus thermophilus Cell Membrane Gene Expression Regulation Bacterial biology.organism_classification Antigenic Variation Molecular biology Fusion protein Lactobacillus Infectious Diseases Biochemistry Genes Bacterial ATP-Binding Cassette Transporters S-layer Bacterial Outer Membrane Proteins |
| Zdroj: | FEMS Microbiology Reviews. 20:47-98 |
| ISSN: | 1574-6976 |
| Popis: | In this chapter we report on the molecular biology of crystalline surface layers of different bacterial groups. The limited information indicates that there are many variations on a common theme. Sequence variety, antigenic diversity, gene expression, rearrangements, influence of environmental factors and applied aspects are addressed. There is considerable variety in the S-layer composition, which was elucidated by sequence analysis of the corresponding genes. In Corynebacterium glutamicum one major cell wall protein is responsible for the formation of a highly ordered, hexagonal array. In contrast, two abundant surface proteins from the S-layer of Bacillus anthracis. Each protein possesses three S-layer homology motifs and one protein could be a virulence factor. The antigenic diversity and ABC transporters are important features, which have been studied in methanogenic archaea. The expression of the S-layer components is controlled by three genes in the case of Thermus thermophilus. One has repressor activity on the S-layer gene promoter, the second codes for the S-layer protein. The rearrangement by reciprocal recombination was investigated in Campylobacter fetus. 7-8 S-layer proteins with a high degree of homology at the 5' and 3' ends were found. Environmental changes influence the surface properties of Bacillus stearothermophilus. Depending on oxygen supply, this species produces different S-layer proteins. Finally, the molecular bases for some applications are discussed. Recombinant S-layer fusion proteins have been designed for biotechnology. |
| Databáze: | OpenAIRE |
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