Au nanoparticles/hollow molybdenum disulfide microcubes based biosensor for microRNA-21 detection coupled with duplex-specific nuclease and enzyme signal amplification
| Autor: | Hong-Lei Shuai, Lin-Xia Fang, Ke-Jing Huang, Meng-Pei Jia, Ying-Xu Chen |
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| Rok vydání: | 2016 |
| Předmět: |
Biomedical Engineering
Biophysics Nanoparticle Biotin DNA Single-Stranded Metal Nanoparticles Nanotechnology 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences chemistry.chemical_compound Limit of Detection Electrochemistry Humans Disulfides Molybdenum disulfide Detection limit Molybdenum Nuclease biology Chemistry technology industry and agriculture Substrate (chemistry) General Medicine Electrochemical Techniques 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences MicroRNAs Ferrocene Electrode biology.protein Gold 0210 nano-technology Biosensor Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 89(Pt 2) |
| ISSN: | 1873-4235 |
| Popis: | An ultrasensitive electrochemical biosensor for detecting microRNAs is fabricated based on hollow molybdenum disulfide (MoS2) microcubes. Duplex-specific nuclease, enzyme and electrochemical-chemical-chemical redox cycling are used for signal amplification. Hollow MoS2 microcubes constructed by ultrathin nanosheets are synthesized by a facile template-assisted strategy and used as supporting substrate. For biosensor assembling, biotinylated ssDNA capture probes are first immobilized on Au nanoparticles (AuNPs)/MoS2 modified electrode in order to combine with streptavidin-conjugated alkaline phosphatase (SA-ALP). When capture probes hybridize with miRNAs, duplex-specific nuclease cleaves the formative duplexes. At the moment, the biotin group strips from the electrode surface and SA-ALP is incapacitated to attach onto electrode. Then, ascorbic acids induce the electrochemical-chemical-chemical redox cycling to produce electrochemical response in the presence of ferrocene methanol and tris (2-carboxyethyl) phosphine. Under optimum conditions, the proposed biosensor shows a good linear relationship between the current variation and logarithm of the microRNAs concentration ranging from 0.1fM to 0.1pM with a detection limit of 0.086fM (S/N=3). Furthermore, the biosensor is successfully applied to detect target miRNA-21 in human serum samples. |
| Databáze: | OpenAIRE |
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