An immunoglobulin G based chimeric protein induced foot-and-mouth disease specific immune response in swine
| Autor: | W.Y. Yan, Z. T. Sheng, L.Q Zhu, H. T. Wong, Z.X. Zheng, Yong Xie, Samuel Chak-Sum Cheng, E. W. C. Chan |
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| Rok vydání: | 2000 |
| Předmět: |
Cellular immunity
Swine Recombinant Fusion Proteins T-Lymphocytes In Vitro Techniques Antibodies Viral Lymphocyte Activation Epitope Virus Immunoglobulin G Microbiology Epitopes Mice Aphthovirus Neutralization Tests Animals Antigens Viral DNA Primers General Veterinary General Immunology and Microbiology biology Base Sequence Viral Vaccine Immunogenicity Public Health Environmental and Occupational Health Viral Vaccines biology.organism_classification Virology Infectious Diseases Foot-and-Mouth Disease Humoral immunity biology.protein Molecular Medicine |
| Zdroj: | Vaccine. 19(4-5) |
| ISSN: | 0264-410X |
| Popis: | Epitopes containing the residues 141aa-160aa and 200aa-213aa from foot-and-mouth disease (FMD) serotype O1K HK type FMDV VP1 were joined to a swine immunoglobulin G single heavy chain constant region (scIgG), creating a novel chimeric protein, named F1-scIgG. In this study, inoculation with F1-scIgG induced both FMD virus-neutralizing antibody response and T cell response in swine. Antisera from these F1-scIgG-inoculated swine protected suckling mice against 1000 lethal dose 50 (1000LD(50)) FMD challenge. F1-scIgG-inoculated swine were also fully protected against 50LD(50) FMD virus challenge. The present study demonstrates the clear potential for viral epitopes linked with self-Ig in novel FMD vaccine design. |
| Databáze: | OpenAIRE |
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