GAL4/GFP enhancer-trap lines for identification and manipulation of cells and tissues in developing Arabidopsis leaves
| Autor: | Nguyen Manh Linh, Enrico Scarpella, Anmol Krishna, Priyanka Govindaraju, Brindhi Amalraj, Sree Janani Ravichandran, Dhruv Lavania |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
0106 biological sciences Green Fluorescent Proteins Arabidopsis Leaf water 01 natural sciences Green fluorescent protein 03 medical and health sciences 0302 clinical medicine Gene Expression Regulation Plant Auxin Arabidopsis thaliana Enhancer trap Gene Leaf development 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology fungi food and beverages Plants Genetically Modified biology.organism_classification Cell biology Plant Leaves Enhancer Elements Genetic 030104 developmental biology Place Cells chemistry Identification (biology) Developmental biology 030217 neurology & neurosurgery Developmental Biology 010606 plant biology & botany |
| DOI: | 10.1101/801357 |
| Popis: | Background Understanding developmental processes requires the unambiguous identification of cells and tissues, and the selective manipulation of the properties of those cells and tissues. Both requirements can most efficiently be satisfied through the use of GAL4/GFP enhancer-trap lines. No such lines, however, have been characterized for the study of early leaf development in the Columbia-0 reference genotype of Arabidopsis. Results Here we address this limitation by identifying and characterizing a set of GAL4/GFP enhancer-trap lines in the Columbia-0 background for the specific labeling of cells and tissues during early leaf development, and for the targeted expression of genes of interest in those cells and tissues. Conclusions By using one line in our set to address outstanding questions in leaf vein patterning, we show that these lines can be used to address key questions in plant developmental biology. |
| Databáze: | OpenAIRE |
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