The transcription factor scleraxis differentially regulates gene expression in tenocytes isolated at different developmental stages
| Autor: | Frances Henson, Deborah J. Guest, N. Evans, Yasmin Z Paterson, S. Kan, Adam P. Cribbs |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Embryology
Biology Tendons Transcriptome Extracellular matrix 03 medical and health sciences 0302 clinical medicine Tendon Injuries Gene expression Basic Helix-Loop-Helix Transcription Factors Animals Horses RNA Messenger RNA-Seq Transcription factor 030304 developmental biology Regulation of gene expression Wound Healing 0303 health sciences Gene knockdown Scleraxis Extracellular Matrix Cell biology Tenocytes Gene Expression Regulation Collagen Chromatin immunoprecipitation 030217 neurology & neurosurgery Transcription Factors Developmental Biology |
| Zdroj: | Mechanisms of Development. 163:103635 |
| ISSN: | 0925-4773 |
| DOI: | 10.1016/j.mod.2020.103635 |
| Popis: | The transcription factor scleraxis (SCX) is expressed throughout tendon development and plays a key role in directing tendon wound healing. However, little is known regarding its role in fetal or young postnatal tendons, stages in development that are known for their enhanced regenerative capabilities. Here we used RNA-sequencing to compare the transcriptome of adult and fetal tenocytes following SCX knockdown. SCX knockdown had a larger effect on gene expression in fetal tenocytes, affecting 477 genes in comparison to the 183 genes affected in adult tenocytes, indicating that scleraxis-dependent processes may differ in these two developmental stages. Gene ontology, network and pathway analysis revealed an overrepresentation of extracellular matrix (ECM) remodelling processes within both comparisons. These included several matrix metalloproteinases, proteoglycans and collagens, some of which were also investigated in SCX knockdown tenocytes from young postnatal foals. Using chromatin immunoprecipitation, we also identified novel genes that SCX differentially interacts with in adult and fetal tenocytes. These results indicate a role for SCX in modulating ECM synthesis and breakdown and provide a useful dataset for further study into SCX gene regulation. |
| Databáze: | OpenAIRE |
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