Identification of hsa-miR-1275 as a Novel Biomarker Targeting MECP2 for Human Epilepsy of Unknown Etiology
| Autor: | Enque Biniam Tesfaye, Liang-Liang Cai, Chi-Meng Tzeng, Hui-Ling Wang, Hsin-Chih Lai, Qing Lin, Ye Zhao, Fanrong Meng, Cong-Xia Lu |
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| Rok vydání: | 2020 |
| Předmět: |
epilepsy of unknown etiology
0301 basic medicine lcsh:QH426-470 Biology MECP2 03 medical and health sciences Epilepsy 0302 clinical medicine hsa-miR-1275 hsa-miR-132 microRNA Genetics medicine TaqMan lcsh:QH573-671 Molecular Biology lcsh:Cytology Gene ontology medicine.disease body regions lcsh:Genetics 030104 developmental biology Apoptosis 030220 oncology & carcinogenesis embryonic structures Etiology Cancer research Gene chip analysis Molecular Medicine |
| Zdroj: | Molecular Therapy: Methods & Clinical Development, Vol 19, Iss, Pp 398-410 (2020) |
| ISSN: | 2329-0501 |
| DOI: | 10.1016/j.omtm.2020.10.005 |
| Popis: | Epilepsy affects around 70 million people worldwide, with a 65% rate of unknown etiology. This rate is known as epilepsy of unknown etiology (EUE). Dysregulation of microRNAs (miRNAs) is recognized to contribute to mental disorders, including epilepsy. However, miRNA dysregulation is poorly understood in EUE. Here, we conducted miRNA expression profiling of EUE by microarray technology and identified 57 pathogenic changed miRNAs with significance. The data and bioinformatic analysis results indicated that among these miRNAs, hsa-microRNA (miR)-1275 was highly associated with neurological disorders. Subsequently, new samples of serum and cerebrospinal fluid were collected for validation of hsa-miR-1275 expression by TaqMan assays. Results show that hsa-miR-1275 in serums of EUE were increased significantly, but in cerebrospinal fluid, the miRNA was decreased. Moreover, the MECP2 gene was selected as a hsa-miR-1275 target based on target prediction tools and gene ontology analysis. Validation of in vitro tests proved that MECP2 expression was specifically inhibited by hsa-miR-1275. Additionally, overexpression of hsa-miR-1275 can elevate expression of nuclear factor κB (NF-κB) and promote cell apoptosis. Taken together, hsa-miR-1275 might represent a novel biomarker targeting MECP2 for human EUE. |
| Databáze: | OpenAIRE |
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