Oxygen consumption of human blood platelets. I. Effect of thrombin
| Autor: | Sidney M. Wolfe, Joseph Muenzer, Eugene C. Weinbach |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Blood Platelets
Male medicine.medical_specialty Time Factors Respiratory rate Biophysics chemistry.chemical_element Stimulation Biochemistry Oxygen law.invention Oxygen Consumption Thrombin law Internal medicine Respiration medicine Humans Platelet Clark electrode Human blood Chemistry Cell Biology Kinetics Endocrinology medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Bioenergetics. 376:237-242 |
| ISSN: | 0005-2728 |
| Popis: | The effect of thrombin on the oxygen consumption of washed human platelets was measured polarographically with the Clark oxygen electrode. The average basal respiratory rate was 18 plus or minus 1.6 (mean plus or minus S.E.) natoms oxygen per min per 10-9 platelets. Thrombin (1.9 units/ml) caused a 4-13-fold increase in the rate of oxygen consumption (138 plus or minus 14 (mean plus or minus S.E.) natoms oxygen per min per 10-9 platelets). The thrombin-stimulated increase of oxygen consumption was transient, lasting from 1 to 1.5 min before returning to the respiratory rate observed before the thrombin addition. Release of platelet constituents appeared to precede the stimulation of oxygen consumption. These results may provide a basis for explaining the discrepancy in the literature concerning the effects of thrombin on platelet respiration. |
| Databáze: | OpenAIRE |
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