A robust lentiviral pseudotype neutralisation assay for in-field serosurveillance of rabies and lyssaviruses in Africa
| Autor: | Tiziana Lembo, Trudy Goddard, Daniel L. Horton, Anthony R. Fooks, Robin A. Weiss, Louis Hendrik Nel, Suzanne McNabb, Sarah Cleaveland, Edward Wright |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Antibodies Viral medicine.disease_cause Tanzania Pseudotype Blood serum Viral Envelope Proteins Seroepidemiologic Studies Dog Diseases Mononegavirales 0303 health sciences biology 3. Good health Infectious Diseases Duvenhage virus Molecular Medicine Female Lagos bat virus Rabies Mokola virus Sensitivity and Specificity Article 03 medical and health sciences Dogs Neutralization Tests Immunology and Microbiology(all) medicine Animals Lyssavirus 030304 developmental biology General Veterinary General Immunology and Microbiology 030306 microbiology Lentivirus Rabies virus Public Health Environmental and Occupational Health biology.organism_classification medicine.disease Virology veterinary(all) Rabies Vaccines Africa |
| Zdroj: | Vaccine Wright, E, McNabb, S, Goddard, T, Horton, D L, Lembo, T, Nel, L H, Weiss, R A, Cleaveland, S & Fooks, A R 2009, ' A robust lentiviral pseudotype neutralisation assay for in-field serosurveillance of rabies and lyssaviruses in Africa ', Vaccine, vol. 27, no. 51, pp. 7178-7186 . https://doi.org/10.1016/j.vaccine.2009.09.024 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2009.09.024 |
| Popis: | The inflexibility of existing serological techniques for detection of rabies in surveillance constrains the benefit to be gained from many current control strategies. We analysed 304 serum samples from Tanzanian dogs for the detection of rabies antibodies in a pseudotype assay using lentiviral vectors bearing the CVS-11 envelope glycoprotein. Compared with the widely used gold standard fluorescent antibody virus neutralisation assay, a specificity of 100% and sensitivity of 94.4% with a strong correlation of antibody titres (r = 0.915) were observed with the pseudotype assay. To increase the assay's surveillance specificity in Africa we incorporated the envelope glycoprotein of local viruses, Lagos bat virus, Duvenhage virus or Mokola virus and also cloned the lacZ gene to provide a reporter element. Neutralisation assays using pseudotypes bearing these glycoproteins reveal that they provide a greater sensitivity compared to similar live virus assays and will therefore allow a more accurate determination of the distribution of these highly pathogenic infections and the threat they pose to human health. Importantly, the CVS-11 pseudotypes were highly stable during freeze-thaw cycles and Storage at room temperature. These results suggest the proposed pseudotype assay is a suitable option for undertaking lyssavirus serosurveillance in areas most affected by these infections. (C) 2009 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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