Lactosamine modulates the rate of migration of GnRH neurons during mouse development
| Autor: | Timothy R. Henion, Denitza Raitcheva, Elizabeth P. Bless, Gerald A. Schwarting, Stuart A. Tobet |
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| Rok vydání: | 2006 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Vomeronasal organ Down-Regulation Cell Count Gonadotropin-releasing hormone Biology N-Acetylglucosaminyltransferases Gonadotropin-Releasing Hormone Mice Prosencephalon Olfactory Mucosa Cell Movement Internal medicine medicine Animals Progenitor cell Mice Knockout Neurons General Neuroscience Embryogenesis Gene Expression Regulation Developmental Amino Sugars Cell Differentiation Cell migration Olfactory Pathways Immunohistochemistry Olfactory Bulb Embryonic stem cell Mice Inbred C57BL Endocrinology medicine.anatomical_structure nervous system Forebrain Female Vomeronasal Organ Olfactory epithelium hormones hormone substitutes and hormone antagonists |
| Zdroj: | European Journal of Neuroscience. 24:654-660 |
| ISSN: | 1460-9568 0953-816X |
| DOI: | 10.1111/j.1460-9568.2006.04955.x |
| Popis: | Gonadotropin-releasing hormone (GnRH) neurons are derived from progenitor cells in the olfactory placodes and migrate from the vomeronasal organ (VNO) across the cribriform plate into the forebrain. At embryonic day (E)12 in the mouse most of these neurons are still in the nasal compartment but by E15 most GnRH neurons have migrated into the forebrain. Glycoconjugates with carbohydrate chains containing terminal lactosamine are expressed by neurons in the main olfactory epithelium and in the VNO. One of the key enzymes required to regulate the synthesis and expression of lactosamine, beta1,3-N-acetylglucosaminyltransferase-1 (beta3GnT1), is strongly expressed by neurons in the olfactory epithelium and VNO, and on neurons migrating out of the VNO along the GnRH migratory pathway. Immunocytochemical analysis of lactosamine and GnRH in embryonic mice reveals that the percentage of lactosamine+-GnRH+ double-labeled neurons decreases from > 80% at E13, when migration is near its peak, to approximately 30% at E18.5, when most neurons have stopped migrating. In beta3GnT1-/- mice, there is a partial loss of lactosamine expression on GnRH neurons. Additionally, a greater number of GnRH neurons were retained in the nasal compartment of null mice at E15 while fewer GnRH neurons were detected later in embryonic development in the ventral forebrain. These results suggest that the loss of lactosamine on a subset of GnRH neurons impeded the rate of migration from the nose to the brain. |
| Databáze: | OpenAIRE |
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