Protective Effect of Nigella Sativa in an Animal Model of Colon Anastomosis With Ischemia/Reperfusion Injury
| Autor: | İlhan Bali, Fatin Rüştü Polat, Mehmet Aziret, Ahmet Koç, Bulent Bilir, Selim Sözen, Cem Oruç, Ufuk Coşkunkan, Seyfi Emir |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Anastomosis
Nigella sativa Ischemia Ischemia/reperfusion injury 030230 surgery 03 medical and health sciences Tissue-Damage 0302 clinical medicine Animal model Anastomotic leaks Medicine Thymoquinone Ischemia-Reperfusion Injury Mucosal Lesion Modulation business.industry Prevention medicine.disease Oil 030220 oncology & carcinogenesis Anesthesia Surgery business Leakage Reperfusion injury Colon anastomosis |
| Zdroj: | International Surgery. 103:139-148 |
| ISSN: | 2520-2456 0020-8868 |
| Popis: | Objective: Anastomotic leaks are one of the chief complications after gastrointestinal surgery. The aim of this study was to evaluate whether Nigella sativa administration protects against ischemia/reperfusion injury on healing of colonic anastomosis in rats. Method: Thirty male Wistar albino rats, weighing between 200 and 240 g, were used in the study. They were randomly divided into three groups (n = 10 for each group): Anastomosis (group 1), anastomosis and ischemia/reperfusion injury (group 2), and treatment group of anastomosis, ischemia/reperfusion injury, and Nigella sativa (group 3). After 7 days, serum, plasma, and colonic tissue were obtained and then all rats were sacrificed. Tissue and serum level of total oxidant status, total antioxidant status, total thiol levels, hydroxyproline, interleukin-6, and TNF-alpha were determined and specimens were histopathologically evaluated. Results: In the Nigella sativa treated rats, serum hydroxyproline levels were significantly higher, while tissue levels were significantly lower than those seen in group 1 and group 2 (P = 0.007, P = 0.01, respectively). In the Nigella sativa group, the serum levels of TNF-α were significantly lower than those seen in group 1 and 2 (P = 0.001). Also, in group 3, the tissue IL-6 level was significantly higher than that seen in group 1 and group 2 (P = 0.009). The histopathologic analysis showed less edema and inflammatory cell infiltration in the Nigella sativa treated group, as well as a statistically significant difference according to the Chiu classification (P < 0.05). Conclusion: The results of this study indicate that Nigella sativa has a protective and therapeutic effect against ischemia/reperfusion injury on the healing of colonic anastomosis in rats. |
| Databáze: | OpenAIRE |
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