Cytokines and lipopolysaccharide induce nitric oxide synthase in cultured rat pulmonary artery smooth muscle
| Autor: | D K, Nakayama, D A, Geller, C J, Lowenstein, H D, Chern, P, Davies, B R, Pitt, R L, Simmons, T R, Billiar |
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| Rok vydání: | 1992 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lipopolysaccharides Male Lipopolysaccharide medicine.medical_treatment Clinical Biochemistry Pulmonary Artery Muscle Smooth Vascular Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Gene expression medicine Animals Northern blot RNA Messenger Nitrite Cycloheximide Molecular Biology Cells Cultured biology Cell Biology DNA Blotting Northern Molecular biology Rats Nitric oxide synthase Cytokine chemistry Biochemistry Enzyme Induction biology.protein Cytokines Tumor necrosis factor alpha Amino Acid Oxidoreductases Nitric Oxide Synthase DNA Probes |
| Zdroj: | American journal of respiratory cell and molecular biology. 7(5) |
| ISSN: | 1044-1549 |
| Popis: | In the current study, we describe cytokine and Escherichia coli lipopolysaccharide (LPS) induction of nitric oxide (NO) synthase mRNA levels in cultured smooth muscle from rat pulmonary artery (RPASM). Exposure of RPASM to interleukin-1 beta, interferon-gamma, or LPS alone did not significantly affect NO synthesis, as determined by nitrite concentrations in media. Exposure to tumor necrosis factor-alpha caused a modest (2x) increase in nitrite production. In contrast, exposure to a combination of the above three cytokines and LPS caused a large increase in NO synthesis. Exposure of RPASM to this combination caused an increase in mRNA levels of NO synthase (as described by Northern blot analysis with 32P-cDNA probe to an inducible form of NO synthase present in murine macrophages) that was apparent as early as 4 h. Expression of the induced gene product after exposure to the cytokine and LPS mixture was evident by significant increases in nitrite production at 12 h. Production of nitrite was completely abolished in the presence of NG-monomethyl-L-arginine (NMA), and this inhibition was reversible by the addition of excess L-arginine. NO synthase mRNA levels were not affected by NMA. The nitrite production induced by the combination of cytokines and LPS was abolished by pretreating cells with cycloheximide. These data indicate that a combination of cytokines and LPS affect expression of the gene for the inducible form of NO synthase in cultured RPASM. |
| Databáze: | OpenAIRE |
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