The effects of jatrorrhizine on contractile responses of rat ileum
Autor: | Zhibi Hu, Jianye Yuan, Jianqun Xie, Dazheng Wu, Guang Ji, Jiyan Zhou |
---|---|
Rok vydání: | 2011 |
Předmět: |
Male
Jatrorrhizine medicine.medical_specialty Berberine Ileum Muscarinic Antagonists In Vitro Techniques Biology chemistry.chemical_compound Internal medicine Muscarinic acetylcholine receptor medicine Animals Drug Interactions Calcium Signaling Rats Wistar Acetylcholine receptor Pharmacology Ryanodine receptor Muscle Smooth Receptors Muscarinic Small intestine Rats medicine.anatomical_structure Endocrinology chemistry Receptors Serotonin Hexamethonium Serotonin Antagonists Acetylcholine Muscle Contraction medicine.drug |
Zdroj: | European Journal of Pharmacology. 663:74-79 |
ISSN: | 0014-2999 |
Popis: | This study was designed to evaluate the effect of jatrorrhizine on smooth muscle contractions isolated from rat ileum longitudinal muscles. Jatrorrhizine increased the amplitude of spontaneous contractions of ileum longitudinal muscles in concentration-dependent manner with an EC(50) of 30.0±8.4μM. Preincubation of ileum strips with atropine (1μM), 4-diphenyllacetoxy-N (2-chloriethyl)-piperidine (4-DAMP, 1μM) or darifenacin (1μM) significantly inhibited acetylcholine (0.1μM)- and jatrorrhizine (100μM)-induced ileum longitudinal muscle contractions, whereas they were not affected by AF-DX116 (1μM) or hexamethonium (100μM). Pretreatment with SB204070 (1μM) rather than 3-tropanyl-indole-3-carboxyleat (tropisetron, 1μM) significantly inhibited 5-HT (10μM)-induced ileum longitudinal muscle contractions. In contrast, jatrorrhizine-induced ileum longitudinal muscle contractions were not inhibited by tropisetron or SB204070. Furthermore, jatrorrhizine-induced ileum longitudinal muscle contractions were strongly inhibited by nifedipine (1μM), and also attenuated by removal of extracellular Ca(2+), U73122 (1μM), ruthenium red (50μM) or 2-aminoethoxydiphenylborate (2-APB, 10μM). Taken together, jatrorrhizine-elicited spontaneous contractions in rat ileum longitudinal muscles are mediated by activation of acetylcholine receptors, mostly the M(3) receptor. Ca(2+) influx through L-type Ca(2+) channel is significantly contributed to jatrorrhizine-elicited spontaneous contractions, and Ca(2+) release via IP(3) and ryanodine pathways are also involved. |
Databáze: | OpenAIRE |
Externí odkaz: |