Analysis of phospholipids in infant formulas using high performance liquid chromatography-tandem mass spectrometry
| Autor: | Carmen Norris, Lin Ma, Bertram Fong |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Phosphatidylserines
Tandem mass spectrometry Mass spectrometry Phosphatidylinositols High-performance liquid chromatography chemistry.chemical_compound Tandem Mass Spectrometry Phosphatidylcholine Humans Chromatography High Pressure Liquid Phospholipids Phosphatidylethanolamine chemistry.chemical_classification Chromatography Chemistry Phosphatidylethanolamines Fatty Acids Fatty acid Infant Reproducibility of Results General Chemistry Phosphatidylserine Infant Formula Sphingomyelins Phosphatidylcholines lipids (amino acids peptides and proteins) General Agricultural and Biological Sciences Sphingomyelin |
| Zdroj: | Journal of agricultural and food chemistry. 61(4) |
| ISSN: | 1520-5118 |
| Popis: | Phospholipids have been used widely in the food industry as emulsifiers, but it is their biological and nutritional function that has been the focus over recent years. The recognition of the importance of phospholipids and sphingomyelin for infant development has led to an increase in the number of infant formulas claiming to contain these complex lipid components. Therefore, the ability to measure these lipids in infant formulas and dairy-derived complex lipid ingredients for fortification purposes is important. A high-performance liquid chromatography-tandem mass spectrometry method that quantifies phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and sphingomyelin found in infant formulas and dairy-derived complex lipid ingredients is described. The method uses external standards of similar fatty acid profile for calibration. The recovery of phospholipids ranged from 92% to 102% with a method repeatability coefficient of variation of 6-10%. In addition to the specificity and selectivity of the method, details of the molecular species in the individual phospholipid classes are available using this method. |
| Databáze: | OpenAIRE |
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