Saccharospirillum mangrovi sp. nov., a bacterium isolated from mangrove sediment
| Autor: | Ye Yuan, Lijian Ding, Dengquan Su, Xiaojun Yan, Shan He, Min Wu, Weiyan Zhang, Slava S. Epstein |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine China Geologic Sediments Ubiquinone Microbiology 03 medical and health sciences Glycolipid RNA Ribosomal 16S Genotype Botany Gene Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Base Composition Phylogenetic tree biology Fatty Acids Nucleic Acid Hybridization Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology Taxonomy (biology) Avicennia Impatiens Gammaproteobacteria Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 68:2813-2818 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | A Gram-negative, spirilla, non-spore-forming, motile and strictly aerobic bacterium, designated HK-33T, was isolated from a mangrove sediment sample in Haikou city, Hainan Province, China. Strain HK-33T was able to grow at 10–45 °C (optimum 37 °C), 0.5–12.0 % (w/v) NaCl (2.0 %, w/v) and pH 5.5–8.5 (pH 7.0). The major cellular fatty acids were C16 : 0 and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). Ubiquinone-8 was the predominant respiratory quinone, and Q-9 was present in trace amounts. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, two unidentified glycolipids, an unidentified aminophosphoglycolipid and two unidentified lipids. The DNA G+C content was 57.3 mol%. According to 16S rRNA gene sequences similarity, strain HK-33T shared 98.6 %, 96.4%, 95.7 and 94.9 % sequence similarities to the species Saccharospirillum correiae CPA1T, Saccharospirillum impatiens EL-105T, Saccharospirillum salsuginis YIM-Y25T and Saccharospirillum aestuarii IMCC 4453T, respectively. Phylogenetic analysis showed that strain HK-33T was clustered with S. correiae CPA1T, S. impatiens EL-105T, S. salsuginis YIM-Y25T and S. aestuarii IMCC 4453T. Results of DNA–DNA hybridization analysis revealed that strain HK-33T shared 36.3±1.7 % DNA relatedness with S. correiae CPA1T. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain HK-33T is considered to represent a novel species in the genus Saccharospirillum , for which the name Saccharospirillum mangrovi sp. nov. is proposed. The type strain is HK-33T (=KCTC 62178T=MCCC 1K03440T). |
| Databáze: | OpenAIRE |
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