In vitro susceptibility of CD4+ and CD8+ T cell subsets to fludarabine
| Autor: | Carlos M. Galmarini, Paula Fernández-Calotti, Jorge Geffner, Julio César Sánchez-Ávalos, Mirta Giordano, Lars Petter Jordheim, Charles Dumontet, Romina Gamberale |
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| Rok vydání: | 2003 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male CIENCIAS MÉDICAS Y DE LA SALUD Cell Survival T cell CD4-CD8 Ratio Apoptosis Medicina Clínica CD8-Positive T-Lymphocytes Nucleoside transporter Biochemistry Peripheral blood mononuclear cell Statistics Nonparametric T LYMPHOCYTE SUBSETS Leukemia B-Cell medicine Humans Cytotoxic T cell Cells Cultured Aged Aged 80 and over Pharmacology PURINE NUCLEOSIDES biology T lymphocyte Deoxycytidine kinase Middle Aged Molecular biology APOPTOSIS medicine.anatomical_structure Chronic Disease Immunology biology.protein Female Medicina Critica y de Emergencia FLUDARABINE LEUKEMIA Immunosuppressive Agents Vidarabine CD8 |
| Zdroj: | Biochemical Pharmacology. 66:2185-2191 |
| ISSN: | 0006-2952 |
| Popis: | Administration of the adenosine analogue fludarabine (FLU) in vivo induces a profound and prolonged T lymphopenia which mainly affects CD4+ cells. To better understand the mechanistic basis underlying this preferential depletion, we analyzed the in vitro susceptibility of T cell subsets to FLU-induced apoptosis. Contrasting with observations in vivo, our results showed that treatment of peripheral blood mononuclear cells with FLU induced a higher level of apoptosis in CD8+ than in CD4+ T lymphocytes. This increased sensitivity of CD8+ T cells to FLU was observed in samples from both, healthy donors and B cell chronic lymphocytic leukemia patients, and resulted in higher CD4:CD8 ratios in FLU-treated than in untreated cultures (P |
| Databáze: | OpenAIRE |
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