S-myotrophin promotes the hypertrophy of skeletal muscle of mice in vivo
| Autor: | Yoshihide Ikeuchi, Hisao Iwamoto, Shohei Shiraishi, Tatsumi Ito, Yusuke Sato, Atsushi Haruno, Toshiya Hayashi, Robert G. Cassens, Yoshinori Nakamura, Sunao Mori, Masamichi Sato, Jiro Chikushi |
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| Rok vydání: | 2006 |
| Předmět: |
mdx mouse
medicine.medical_specialty Biochemistry Muscle hypertrophy Dystrophin Mice Myotrophin Laminin Internal medicine medicine Animals Myocyte Muscle Skeletal education Basement membrane education.field_of_study biology Body Weight Skeletal muscle Hypertrophy Cell Biology Muscular Dystrophy Animal Mice Inbred C57BL Endocrinology medicine.anatomical_structure Immunology Mice Inbred mdx biology.protein Intercellular Signaling Peptides and Proteins |
| Zdroj: | The International Journal of Biochemistry & Cell Biology. 38:1114-1122 |
| ISSN: | 1357-2725 |
| Popis: | S-myotrophin is a newly discovered muscle growth factor. Effects of crude S-myotrophin injection on the growth and morphology of skeletal muscle of normal, ScN and mdx mice were investigated in the present study. Total dose of crude S-myotrophin was 100 microg (100 microg protein/ml x 50 microl x 20 times). In the case of normal mice (Sea:ddY), body weight and the weight of M. gluteus major of crude S-myotrophin injected mice was significantly heavier than that of control (PBS-injected) mice after 5 weeks' feeding. Antibody staining of laminin and dystrophin showed clear sarcolemmal and basement membrane structure surrounding each muscle fibre. The numbers of muscle fibres per 100 microm(2) was less in crude S-myotrophin-injected normal mice than in PBS-injected mice. Quite similar observations as in the case of normal mice were obtained in the case of ScN mice having heterogeneous gene of dystrophin. In the case of mdx mice, body weight and the weight of M. gluteus major of crude S-myotrophin injected mdx mice was significantly heavier than that of PBS-injected mdx mice. Antibody staining of laminin showed almost intact structure of the basement membrane containing laminin even in skeletal muscle of mdx mice subjected to crude S-myotrophin injection, while irregular and incompletely developed structure of muscle fibres or necrosis were observed in muscle fibres of PBS-injected mdx mice. In spite of crudeness of the preparation, the present animal experiments indicate that S-myotrophin has a strong growth promoting activity of muscle cells of normal and dystrophic mice. |
| Databáze: | OpenAIRE |
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