Spread of the florfenicol resistance floR gene among clinical Klebsiella pneumoniae isolates in China
Autor: | Junwan Lu, Jinfang Zhang, Peizhen Li, Lei Xu, Pingping Li, Tingyuan Zhu, Qiyu Bao, Yabo Liu, Kewei Li, Wu Zhou, Liyan Ni, Shunfei Lu, Teng Xu, Huiguang Yi, Cong Cheng |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Microbiology (medical) Florfenicol China Klebsiella pneumoniae 030106 microbiology Flor Drug resistance Microbial Sensitivity Tests Plasmid Microbiology lcsh:Infectious and parasitic diseases floR 03 medical and health sciences chemistry.chemical_compound Drug Resistance Bacterial Medicine Humans Pharmacology (medical) lcsh:RC109-216 Gene Phylogeny Comparative genomics Thiamphenicol biology business.industry Research Public Health Environmental and Occupational Health Genomics Sequence Analysis DNA biology.organism_classification Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Klebsiella Infections 030104 developmental biology Infectious Diseases chemistry Genes Bacterial Conjugation Genetic Horizontal gene transfer business Genome Bacterial Human pathogen |
Zdroj: | Antimicrobial Resistance and Infection Control, Vol 7, Iss 1, Pp 1-9 (2018) Antimicrobial Resistance and Infection Control |
ISSN: | 2047-2994 |
Popis: | Background Florfenicol is a derivative of chloramphenicol that is used only for the treatment of animal diseases. A key resistance gene for florfenicol, floR, can spread among bacteria of the same and different species or genera through horizontal gene transfer. To analyze the potential transmission of resistance genes between animal and human pathogens, we investigated floR in Klebsiella pneumoniae isolates from patient samples. floR in human pathogens may originate from animal pathogens and would reflect the risk to human health of using antimicrobial agents in animals. Methods PCR was used to identify floR-positive strains. The floR genes were cloned, and the minimum inhibitory concentrations (MICs) were determined to assess the relative resistance levels of the genes and strains. Sequencing and comparative genomics methods were used to analyze floR gene-related sequence structure as well as the molecular mechanism of resistance dissemination. Results Of the strains evaluated, 20.42% (67/328) were resistant to florfenicol, and 86.96% (20/23) of the floR-positive strains demonstrated high resistance to florfenicol with MICs ≥512 μg/mL. Conjugation experiments showed that transferrable plasmids carried the floR gene in three isolates. Sequencing analysis of a plasmid approximately 125 kb in size (pKP18–125) indicated that the floR gene was flanked by multiple copies of mobile genetic elements. Comparative genomics analysis of a 9-kb transposon-like fragment of pKP18–125 showed that an approximately 2-kb sequence encoding lysR-floR-virD2 was conserved in the majority (79.01%, 83/105) of floR sequences collected from NCBI nucleotide database. Interestingly, the most similar sequence was a 7-kb fragment of plasmid pEC012 from an Escherichia coli strain isolated from a chicken. Conclusions Identified on a transferable plasmid in the human pathogen K. pneumoniae, the floR gene may be disseminated through horizontal gene transfer from animal pathogens. Studies on the molecular mechanism of resistance gene dissemination in different bacterial species of animal origin could provide useful information for preventing or controlling the spread of resistance between animal and human pathogens. Electronic supplementary material The online version of this article (10.1186/s13756-018-0415-0) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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