Lymphangiogenesis in Crohn's disease: an immunohistochemical study using monoclonal antibody D2-40
| Autor: | Pietro Tonelli, S Bartolini, Federica Pedica, Paola Baccarini, C. Ligorio |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Colon Lymphangiectasia Inflammatory bowel disease Pathology and Forensic Medicine lymphatic vessels Antibodies Monoclonal Murine-Derived Crohn Disease Ileum inflammatory bowel disease medicine Humans Molecular Biology Aged Crohn's disease business.industry Antibodies Monoclonal Cell Biology General Medicine Middle Aged medicine.disease Immunohistochemistry lymphangiogenesis Lymphangiogenesis Lymphatic system medicine.anatomical_structure Circulatory system Colitis Ulcerative Female business Blood vessel |
| Popis: | Crohn's disease (CD) is a chronic inflammatory bowel disorder of unknown etiology. An involvement of the intestinal lymphatic system has been suggested. Recently, monoclonal antibodies have become available to distinguish lymphatic vessels from blood vessels. The aim of the study was to examine the distribution of lymphatic vessels in ileal and colic walls of patients affected by CD and compare it with healthy controls and other inflammatory bowel diseases. Twenty-eight cases of CD, 13 cases of other inflammatory bowel diseases, and 10 normal ileal and colic walls were studied. Immunohistochemical staining was performed using the monoclonal antibody D2-40. Quantification of lymphatic vessels was performed by identifying four fields with high density of lymphatics and then counting the number of lymphatic vessels at high resolution. Lymphatic diameter was also evaluated by using an ocular micrometer. Lymphatic vessels showed the highest density in CD specimens. The median number of lymphatics was significantly higher both in ileal and colic samples of CD than the other inflammatory diseases as well as normal controls. Moreover, in patients with CD, diffuse lymphangiectasia was also observed. The present data suggest that lymphangiogenesis and lymphangiectasia probably play a role in the pathogenesis of CD. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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