Bromodeoxyuridine- and cyclic AMP-mediated regulation of tyrosinase in Syrian hamster melanoma cells
| Autor: | Sikha Rauth, Richard L. Davidson, George E. Hoganson |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Melanocyte-stimulating hormone Tyrosinase Melanoma Experimental Hamster Gene Expression Biology In Vitro Techniques chemistry.chemical_compound Cricetulus Internal medicine Cricetinae Genetics medicine Cyclic AMP Tumor Cells Cultured Animals Northern blot Melanocyte-Stimulating Hormones RNA Messenger Monophenol Monooxygenase Pigmentation Cell Biology General Medicine Blotting Northern Endocrinology Mechanism of action chemistry Bromodeoxyuridine Bucladesine Cell culture sense organs medicine.symptom Thymidine |
| Zdroj: | Somatic cell and molecular genetics. 16(6) |
| ISSN: | 0740-7750 |
| Popis: | The thymidine analog 5-bromodeoxyuridine (BrdU) suppresses pigmentation and tyrosinase activity in Syrian hamster melanoma cells W1-1-1. Studies on the molecular mechanism of suppression of pigmentation indicated that BrdU treatment affects the level of tyrosinase gene transcripts. No detectable tyrosinase message was found by Northern blot analysis in cells cultured in the presence of BrdU at concentrations even as low as 0.2 microM. The level of tyrosinase mRNA was found to reflect the level of pigmentation and tyrosinase activity. Studies with dibutyryl cyclic AMP (cAMP) showed that it inhibited pigment synthesis in W1-1-1 cells. With increasing concentrations of cAMP ranging from 10 microM to 300 microM, pigmentation and tyrosinase activity decreased progressively. This inhibition was found to be associated with a corresponding decrease in the level of tyrosinase mRNA. W1-1-1 cells were found not to respond to melanocyte stimulating hormone (MSH). There was no change in pigmentation, tyrosinase activity, or tyrosinase mRNA level in W1-1-1 cells in the presence of MSH. Similarly, theophylline, a phosphodiesterase inhibitor, had no effect on pigmentation or tyrosinase activity in W1-1-1 cells. |
| Databáze: | OpenAIRE |
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