Effects of compound probiotics and aflatoxin-degradation enzyme on alleviating aflatoxin-induced cytotoxicity in chicken embryo primary intestinal epithelium, liver and kidney cells
| Autor: | Qingqiang Yin, Xiao-Fei Hu, Juan Chang, Xiaoxiang Xu, Dang Xiaowei, Liu Chaoqi, Hongwei Guo, Quan-Liang Wang, Wang Ping |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Aflatoxin
Aflatoxin B1 lcsh:Biotechnology Cell Biophysics lcsh:QR1-502 Inflammation Applied Microbiology and Biotechnology lcsh:Microbiology Andrology 03 medical and health sciences lcsh:TP248.13-248.65 medicine Cytotoxicity Cell damage 030304 developmental biology 0303 health sciences Kidney Chemistry Liver cell 030302 biochemistry & molecular biology Compound probiotics Cell damage alleviation medicine.disease Intestinal epithelium Chicken embryo primary cells medicine.anatomical_structure Original Article Mycotoxin-degradation enzyme medicine.symptom |
| Zdroj: | AMB Express, Vol 11, Iss 1, Pp 1-12 (2021) AMB Express |
| ISSN: | 2191-0855 |
| Popis: | Aflatoxin B1 (AFB1) is one of the most dangerous mycotoxins for humans and animals. This study aimed to investigate the effects of compound probiotics (CP), CP supernatant (CPS), AFB1-degradation enzyme (ADE) on chicken embryo primary intestinal epithelium, liver and kidney cell viabilities, and to determine the functions of CP + ADE (CPADE) or CPS + ADE (CPSADE) for alleviating cytotoxicity induced by AFB1. The results showed that AFB1 decreased cell viabilities in dose-dependent and time-dependent manner. The optimal AFB1 concentrations and reactive time for establishing cell damage models were 200 µg/L AFB1 and 12 h for intestinal epithelium cells, 40 µg/L and 12 h for liver and kidney cells. Cell viabilities reached 231.58% (p < 0.05) for intestinal epithelium cells with CP addition, 105.29% and 115.84% (p < 0.05) for kidney and liver cells with CPS additions. The further results showed that intestinal epithelium, liver and kidney cell viabilities were significantly decreased to 87.12%, 88.7% and 84.19% (p < 0.05) when the cells were exposed to AFB1; however, they were increased to 93.49% by CPADE addition, 102.33% and 94.71% by CPSADE additions (p < 0.05). The relative mRNA abundances of IL-6, IL-8, TNF-α, iNOS, NF-κB, NOD1 (except liver cell) and TLR2 in three kinds of primary cells were significantly down-regulated by CPADE or CPSADE addition, compared with single AFB1 group (p < 0.05), indicating that CPADE or CPSADE addition could alleviate cell cytotoxicity and inflammation induced by AFB1 exposure through suppressing the activations of NF-κB, NOD1 and TLR2 pathways. |
| Databáze: | OpenAIRE |
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