Studies on glucagon secretion using isolated islets of langerhans of the rat
| Autor: | Peter H. Wright, V. L. Williams, J. R. Oliver |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Epinephrine Arginine Endocrinology Diabetes and Metabolism medicine.medical_treatment In Vitro Techniques Hypoglycemia Islets of Langerhans Internal medicine Insulin Secretion Internal Medicine medicine Animals Insulin chemistry.chemical_classification geography geography.geographical_feature_category Chemistry Glucagon secretion Glucagon medicine.disease Islet Rats Amino acid Glucose Microbial Collagenase Endocrinology Blood sugar regulation hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Diabetologia. 12:301-306 |
| ISSN: | 1432-0428 0012-186X |
| DOI: | 10.1007/bf00420972 |
| Popis: | Glucagon secretion and its control have been studied in perifused isolated islets of Langerhans of the rat. It was shown that a low concentration of glucose per se does not cause increased glucagon secretion, but that at low glucose concentrations the amino acid arginine stimulates a biphasic secretory response. Such amino acid stimulated glucagon secretion can be suppressed by increasing the glucose content of the perifused media from 1.67 to 5.5 or 16.7 mM; insulin secretion is also then increased. Since high concentrations of added porcine insulin (10 mU/ml) did not affect amino acid stimulated glucagon secretion at low glucose concentration, it was concluded that high concentrations of glucose and not insulin secreted in response to that glucose are probably responsible for suppression of glucagon secretion. At low concentrations of glucose, epinephrine (2.5 X 10(-7) M) also stimulated glucagon secretion. It is concluded that isolated rat islets of Langerhans can be used for the study of glucagon secretion in vitro, and that substances appearing in the blood in vivo at low glucose concentrations are probably responsible for increased glucagon secretion under conditions associated with hypoglycemia. |
| Databáze: | OpenAIRE |
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