Adsorption kinetics of c-Fos and c-Jun to air–water interfaces
| Autor: | Bruno Maggio, Maria Elisabete Darbello Zaniquelli, Thatyane M. Nobre, Graciela A. Borioli, Maximiliano Del Boca |
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| Rok vydání: | 2007 |
| Předmět: |
Chromatography
biology Proto-Oncogene Proteins c-jun Surface Properties Chemistry Air Organic Chemistry Kinetics c-jun Biophysics Water Biological membrane Biochemistry c-Fos Adsorption Adsorption kinetics Chemical engineering Monolayer biology.protein Humans Proto-Oncogene Proteins c-fos Protein adsorption |
| Zdroj: | Biophysical Chemistry. 130:132-138 |
| ISSN: | 0301-4622 |
| DOI: | 10.1016/j.bpc.2007.08.005 |
| Popis: | The kinetics of adsorption to air-water interfaces of the biomembrane active transcription factors c-Fos, c-Jun and their mixtures is investigated. The adsorption process shows three distinct stages: a lag time, a fast pseudo zero-order stage, and a halting stage. The initial stage determines the course of the process, which is concentration dependent until the end of the fast stage. We show that c-Fos has faster adsorption kinetics than c-Jun over all three stages and that the interaction between both proteins is apparent in the adsorption profiles of the mixtures. Protein molecular reorganization at the interface determines the transition to the final adsorption stage of the pure proteins as well as that of the mixtures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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