Long noncoding RNA LBX2-AS1-modulated miR-4766-5p regulates gastric cancer development through targeting CXCL5
Autor: | XiaoHua An, Shuai Kong, Shubo Tian, GuangChuan Wang, Yuezhi Chen, ZeZhong Chen, Tao Xu, Lipan Peng |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Cancer Research
Chemokine miR-4766-5p lcsh:RC254-282 03 medical and health sciences 0302 clinical medicine Genetics lcsh:QH573-671 030304 developmental biology GC 0303 health sciences Reporter gene Gene knockdown biology Cell growth Chemistry lcsh:Cytology RNA CXCL5 lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens LBX2-AS1 Long non-coding RNA Cell biology Antisense RNA Oncology 030220 oncology & carcinogenesis biology.protein Primary Research |
Zdroj: | Cancer Cell International, Vol 20, Iss 1, Pp 1-12 (2020) Cancer Cell International |
ISSN: | 1475-2867 |
Popis: | Background Long noncoding RNAs (LncRNAs) have been reported to critically regulate gastric cancer (GC). Recently, it was reported that LBX2 antisense RNA 1 (LBX2-AS1) is abnormally expressed in GC. However, the role of LBX2-AS1 in the malignancy of GC is worth further discussion. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the LBX2-AS1, miR-4766-5p and C-X-C motif chemokine (CXCL5) expression in GC tissues and cells. Dual-luciferase reporter assay was applied to examine the target relationship between LBX2-AS1 and miR-4766-5p or miR-4766-5p and CXCL5. Cell counting kit-8 (CCK-8) and Transwell assays were used to detect cell proliferation, migration and invasion rates. The protein expression of CXCL5 was confirmed using western blot. The RNA pull down experiment was used to verify the specificity of LBX2-AS1 and miR-4766-5p on BGC-823 and SGC-7901 cells. Results LBX2-AS1 was up-regulated in GC tissues and cells, and its knockdown suppressed proliferation, migration and invasion of GC cells. While, overexpression of LBX2-AS1 increased proliferation and increased CXCL5 mRNA level. CXCL5 improved cell proliferation, migration and invasion of GC cells. LBX2-AS1 could bind to miR-4766-5p to regulate CXCL5 expression. Overexpression of CXCL5 overturned those effects of miR-4766-5p in GC cells. RNA Pull down shown that BGC-823 and SGC-7901 cells, miR-4766-5p specifically binds to LBX2-AS1. Conclusions In short, this study demonstrated that LBX2-AS1 promoted proliferation, migration and invasion through up-regulation CXCL5 mediated by miR-4766-5p in GC. The LBX2-AS1/miR-4766-5p/CXCL5 regulatory axis provides a theoretical basis for the research on lncRNA-directed therapeutics in GC. |
Databáze: | OpenAIRE |
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