Brown adipose tissue mitochondria are cytochrome c ? subsaturated
| Autor: | C. K. Ramakrishna Kurup, T. Ramasarma, Amos Gaikwad |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Hemeprotein Cytochrome Citric Acid Cycle Clinical Biochemistry Adipose tissue Cytochrome c Group Mitochondria Liver Cell Fractionation Oxygen Consumption Adipose Tissue Brown Brown adipose tissue medicine Animals Molecular Biology Binding Sites biology Cytochrome c Rats Inbred Strains Cell Biology General Medicine Electron transport chain Thermogenin Mitochondria Rats Organelle membrane medicine.anatomical_structure Biochemistry Glycerophosphates biology.protein Spectrophotometry Ultraviolet |
| Zdroj: | Molecular and Cellular Biochemistry. 105 |
| ISSN: | 1573-4919 0300-8177 |
| DOI: | 10.1007/bf00227751 |
| Popis: | The oxidative activity of mitochondria freshly isolated from brown adipose tissue of rats was stimulated two-fold on the addition of small concentrations of exogenous cytochrome c to the reaction medium. Loss of membrane-bound cytochrome c did not occur during isolation of mitochondria. Estimation of the high-affinity binding sites on the organelle membrane indicated that less than a third of these sites remained saturated with cytochrome c. The pigment is thus shown to be a functionally limiting electron transport component in brown adipose tissue. |
| Databáze: | OpenAIRE |
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