Projection length stimulated by oxytocin is modulated by the inhibition of calcium signaling in U-87MG cells
Autor: | Alexandra Reichova, Ana M. Castejon, Daniela Ostatníková, Zuzana Lestanova, Alexander Kiss, Zuzana Bacova, Mohammed M. Alanazi, Martina Zatkova, F. Puerta, Jan Bakos |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
medicine.drug_class chemistry.chemical_element Calcium channel blocker Calcium Oxytocin Calcium in biology Cell Line 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Humans Calcium Signaling Estrenes Cell Shape Biological Psychiatry Calcium signaling Neurons Ryanodine receptor Calcium channel Cilnidipine Oxytocin receptor Pyrrolidinones Cell biology Psychiatry and Mental health 030104 developmental biology Neurology chemistry Astrocytes Quercetin Neurology (clinical) 030217 neurology & neurosurgery medicine.drug |
Zdroj: | Journal of Neural Transmission. 125:1847-1856 |
ISSN: | 1435-1463 0300-9564 |
Popis: | Neuropeptide oxytocin contributes to the regulation of glial cell morphology. The precise mechanisms, however, are not yet fully understood. In the present study, we have investigated whether an oxytocin-induced increase of intracellular calcium is required for cell extension in astrocyte-like U-87MG cells. Oxytocin (1 µM) significantly increased the length of the cell projections measured by the green-fluorescent protein labeled microtubule-associated protein after 48 h. The knockdown of oxytocin receptors (OXTR) in U-87MG cells prevented the elongation of the projections. Incubation of U-87MG cells in the presence of oxytocin, resulted in a significant increase of intracellular calcium, specifically blocked by the OXTR antagonist L-371,257. Both quercetin, which is a phosphoinositide 3-kinase inhibitor, and the phospholipase C inhibitor U-73122 reduced oxytocin-induced elevation of intracellular calcium concentration. Conversely, neither diltiazem, an L-type voltage-gated calcium channel blocker nor tetracaine, which is a blocker of the ryanodine receptors, showed an effect on intracellular calcium levels. Treatment of cells with quercetin, U-73122 and the voltage-gated calcium channel blockers cilnidipine, ω-agatoxin and mibefradil prevented the elongation of projections stimulated by oxytocin. Oxytocin treatment resulted in a significant increase in gene and protein expression of the scaffolding protein SHANK3. Our results clearly show that activation of OXTRs contributes to the elongation of cell projections in astrocyte-like U-87MG cells and that this effect is mediated by an extracellular calcium influx accompanied by an increase in scaffolding proteins expression. |
Databáze: | OpenAIRE |
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