Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid
| Autor: | Nuojin Wang, Qiu Zhang, Yunxia Lu, Fang Dai, Li Chen, Li Gui, Guanjun Chen, Ting Wang, Zhaohui Fang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Endocrinology Diabetes and Metabolism Palmitic Acid Adipokine Adipose tissue 030209 endocrinology & metabolism Inflammation Resveratrol Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Endocrinology Insulin resistance Adipokines Internal medicine 3T3-L1 Cells Stilbenes medicine Adipocytes Animals Chemistry Endoplasmic reticulum Cell Differentiation medicine.disease Endoplasmic Reticulum Stress Lipids 030104 developmental biology Adipose Tissue medicine.symptom Adipocyte hypertrophy Insulin Resistance |
| Zdroj: | Endocrine. 55(3) |
| ISSN: | 1559-0100 |
| Popis: | This study aimed to determine whether resveratrol treatment alleviates endoplasmic reticulum stress and changes the expression of adipokines in adipose tissues and cells. 8-week-old male C57BL/6 mice were fed a high-calorie diet (HCD group) or high-calorie diet supplemented with resveratrol (high-calorie diet + resveratrol group) for 3 months. Insulin resistance, serum lipids and proinflammatory indices, the size and inflammatory cell infiltration in subcutaneous and visceral adipose tissues were analyzed. The gene expressions of endoplasmic reticulum stress, adipokines, and inflammatory cytokines were determined. The induced mature 3T3-L1 cells were pretreated with resveratrol and then palmitic acid, and the gene expressions of endoplasmic reticulum stress, adipokines, and inflammatory cytokines were determined. Subcutaneous and visceral adipose tissues in the high-calorie diet-fed mice exhibited adipocyte hypertrophy, inflammatory activation, and endoplasmic reticulum stress. Resveratrol alleviated high-calorie diet-induced insulin resistance and endoplasmic reticulum stress, increased expression of SIRT1, and reversed expression of adipokines in varying degrees in both subcutaneous and visceral adipose tissues. The effects of resveratrol on palmitic acid-treated adipocytes were similar to those shown in the tissues. Resveratrol treatment obviously reversed adipocyte hypertrophy and insulin resistance by attenuating endoplasmic reticulum stress and inflammation, thus increasing the expression of SIRT1 and inverting the expression of adipokines in vivo and in vitro. |
| Databáze: | OpenAIRE |
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