Expressed sequence tags identify human isologs of the ARF-dependent phospholipase D
| Autor: | Pierre Pontarotti, Hugues Chap, Michel Record, J.P. Henry, Clotilde Cariven, Gérard Ribbes |
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| Rok vydání: | 1996 |
| Předmět: |
Reading Frames
Databases Factual Molecular Sequence Data Biophysics Saccharomyces cerevisiae Biology Phospholipase Biochemistry GTP-Binding Proteins Complementary DNA Consensus Sequence medicine Phospholipase D Humans Amino Acid Sequence Molecular Biology Conserved Sequence Phylogeny Sequence Tagged Sites chemistry.chemical_classification Expressed sequence tag Human liver Sequence Homology Amino Acid ADP-Ribosylation Factors Brain Oryza Cell Biology Human brain Plants Molecular biology Amino acid Isoenzymes medicine.anatomical_structure chemistry Liver Carrier Proteins Phospholipase D1 Software |
| Zdroj: | Biochemical and biophysical research communications. 224(1) |
| ISSN: | 0006-291X |
| Popis: | By searching into Expressed Sequence Tags databases (dbEST) using Blast X algorithm software and a plant phospholipase D as template, we have identified a cDNA from human brain (Z45777) which encodes for a protein similar to the amino acid region 743-929 of the human phospholipase D1 (PLD1), and a cDNA from human liver (R93485) which encodes for a protein similar to region 815-932 of PLD1. Sequence comparison between cloned phospholipases showed the presence of 3 conserved amino acid sequences: AFVGGIDLAYGRWD (box A), IIGSANINDRS (box B), and YIYIENQFFI (box C). Phylogenic analysis indicated that the cDNA from brain and liver encoded for human isologs of PLD1. |
| Databáze: | OpenAIRE |
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