| Autor: |
Jong-Young Kwak, Joo-In Park, Valentin A. Stonik, Valeria V. Shastina, Jin-Sook Jeong, Jin-Yeong Han, Yong-Woo Na, Sung-Won Shin, Eun-Seon Park, Seong-Hoon Yun |
| Rok vydání: |
2023 |
| DOI: |
10.1158/1078-0432.22445352 |
| Popis: |
PDF file - 2.3MB, Treatment of colorectal cancer cells with STC leads to generation of ceramide through the activation of acid SMase and neutral SMase. (A and B) CT-26 cells (1 �~ 105 cells/well) were incubated with STC in the presence or absence of desipramine or GW4869 for 24 h. After treatment, cells were fixed and permeabilized. Then, samples were stained with FITC-anti-ceramide and (A) PE-anti-acid SMase or (B) PE-anti-neutral SMase antibodies. Left panel shows the low magnifications of CT-26 cells treated as in A. Right panel shows higher magnifications of CT-26 cells treated as in A. (C and D) SNU-C4 cells (1 �~ 105 cells/well) were incubated with STC in the presence or absence of desipramine or GW4869 for 24 h. After treatment, cells were fixed and permeabilized. Then, samples were stained with FITC-anti-ceramide and (C) PE-anti-acid SMase or (D) PE-anti-neutral SMase antibodies. Left panel shows the low magnifications of SNU-C4 cells treated as in C. Right panel shows higher magnifications of SNU-C4 cells treated as in C. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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