Glucose metabolism of malignant cells is not regulated by transketolase-like (TKTL)-1
| Autor: | Arnulf Mayer, Peter Vaupel, Angelika von Wallbrunn |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cancer Research
Blotting Western Clone (cell biology) Transketolase Biology Isozyme Gene Expression Regulation Enzymologic Mice Cell Line Tumor Neoplasms Animals Humans Nuclear protein Tumor hypoxia Oncogene Leiomyoma Antibodies Monoclonal Cell cycle Gene Expression Regulation Neoplastic Glucose Oncology Biochemistry Monoclonal Uterine Neoplasms Cancer research Female HeLa Cells |
| Zdroj: | International journal of oncology. 37(2) |
| ISSN: | 1791-2423 |
| Popis: | An isoenzyme of transketolase, transketolase-like (TKTL)-1, has been hypothesized to play a pivotal role in the pathophysiology of malignant tumors. Available data are based on the detection of the putative TKTL-1 protein with one particular mouse monoclonal anti-TKTL-1 antibody, clone JFC12T10. In this study it was demonstrated that a) JFC12T10 detects multiple unspecific bands in Western blots, b) a 75-kDa band hitherto referred to as TKTL-1 corresponds to a nuclear protein and c) immunohistochemical detection of TKTL-1 in benign leiomyomas yields an expression pattern identical to that found in a variety of malignant tumors. In RT-PCR assays, using three different primer pairs for transketolase, TKTL-1 and yet another isogene of transketolase, TKTL-2, a relevant expression of TKTL-1 was not detectable in any of the 6 malignant tumor cell lines investigated (MCF-7, A549, HeLa, HT1080, M21 and TF-1). Expression levels of TKTL-1 were rather similar to those found for TKTL-2, although the latter has never been implicated in malignant disease. On the basis of these data, nutritional recommendations based on a hypothetically TKTL-1 controlled metabolism of tumor cells must be regarded as lacking scientific evidence. |
| Databáze: | OpenAIRE |
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