Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification

Autor: Hans-Reinhard Hofferbert, Richard M. Twyman, Güven Edgü, Eckhard Tacke, Stefanie Hartje, Jost Muth, Lena Julie Freund, Gundula A. Noll, Dirk Prüfer
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Diagnostic methods
Loop-mediated isothermal amplification
Sensitivity and Specificity
01 natural sciences
Article
Catalysis
Plant Viruses
lcsh:Chemistry
lateral-flow dipstick
Inorganic Chemistry
Crop
03 medical and health sciences
Disease management (agriculture)
Humans
Multiplex
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Plant Diseases
Solanum tuberosum
Sample handling
biology
business.industry
fungi
Organic Chemistry
Reproducibility of Results
food and beverages
General Medicine
Dipstick
biology.organism_classification
Computer Science Applications
Biotechnology
Molecular Typing
Plant Tubers
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Molecular Diagnostic Techniques
Tobacco rattle virus
RNA
Viral
tobacco rattle virus
Colorimetry
business
Nucleic Acid Amplification Techniques
loop-mediated isothermal amplification
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 22
International Journal of Molecular Sciences, Vol 21, Iss 8741, p 8741 (2020)
ISSN: 1422-0067
Popis: Potato is an important staple food crop in both developed and developing countries. However, potato plants are susceptible to several economically important viruses that reduce yields by up to 50% and affect tuber quality. One of the major threats is corky ringspot, which is a tuber necrosis caused by tobacco rattle virus (TRV). The appearance of corky ringspot symptoms on tubers prior to commercialization results in &asymp
45% of the tubers being downgraded in quality and value, while &asymp
55% are declared unsaleable. To improve current disease management practices, we have developed simple diagnostic methods for the reliable detection of TRV without RNA purification, involving minimalized sample handling (mini), subsequent improved colorimetric loop-mediated isothermal amplification (LAMP), and final verification by lateral-flow dipstick (LFD) analysis. Having optimized the mini-LAMP-LFD approach for the sensitive and specific detection of TRV, we confirmed the reliability and robustness of this approach by the simultaneous detection of TRV and other harmful viruses in duplex LAMP reactions. Therefore, our new approach offers breeders, producers, and farmers an inexpensive and efficient new platform for disease management in potato breeding and cultivation.
Databáze: OpenAIRE
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