Effect of Repeated Injection of Iodixanol on Renal Function in Healthy Wistar Rats Using Functional MRI

Autor: Wen-ge Sun, Ke Ren, Yi Liu, Songbai Li, Yongfang Wang, Lizhi Xie
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
medicine.medical_specialty
Time Factors
Article Subject
Urinary system
030232 urology & nephrology
Urology
Renal function
lcsh:Medicine
Kidney
General Biochemistry
Genetics and Molecular Biology
Injections
030218 nuclear medicine & medical imaging
03 medical and health sciences
0302 clinical medicine
Lipocalin-2
In vivo
Proto-Oncogene Proteins
Triiodobenzoic Acids
Renal hypoxia
Image Processing
Computer-Assisted
medicine
Animals
Rats
Wistar
General Immunology and Microbiology
medicine.diagnostic_test
business.industry
lcsh:R
Acute kidney injury
Magnetic resonance imaging
General Medicine
Hypoxia-Inducible Factor 1
alpha Subunit
medicine.disease
Magnetic Resonance Imaging
Iodixanol
Lipocalins
Oxygen
Diffusion Magnetic Resonance Imaging
Creatinine
business
Acute-Phase Proteins
Research Article
Diffusion MRI
medicine.drug
Zdroj: BioMed Research International, Vol 2018 (2018)
BioMed Research International
ISSN: 2314-6141
2314-6133
Popis: Purpose. To determine the optimal time interval of repeated intravenous injections of iodixanol in rat model and to identify the injury location and causes of renal damage in vivo. Materials and Methods. Rats were randomly divided into Control group, Group 1 with one iodixanol injection, and Group 2 with two iodixanol injections. Group 2 was subdivided into 3 cohorts according to the interval between the first and second iodixanol injections as 1, 3, and 5 days, respectively. Blood oxygen level-dependent (BOLD) imaging and diffusion weighted imaging (DWI) were performed at 1 hour, 1 day, 3 days, 5 days, and 10 days after the application of solutions. Results. Compared with Group 1 (7.2%), Group 2 produced a remarkable R2⁎ increment at the inner stripe of the renal outer medulla by 15.37% (P=0.012), 14.83% (P=0.046), and 13.53% (P>0.05), respectively, at 1 hour after repeated injection of iodixanol. The severity of BOLD MRI to detect renal hypoxia was consistent with the expression of HIF-1α and R2⁎ was well correlated with HIF-1α expression (r=0.704). The acute tubular injury was associated with urinary NGAL and increased significantly at 1 day. Conclusions. Repetitive injection of iodixanol within a short time window can induce acute kidney injury, the impact of which on renal damage in rats disappears gradually 3–5 days after the injections.
Databáze: OpenAIRE
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