Increased HE4 mRNA Expression Correlates with High Level Of eIF3a mRNA And Better Survival in Women with Epithelial Ovarian Cancer
| Autor: | Hong Hao Zhou, Yan Chen, Yu Xi Tang, Chen Hui Luo, Min Zhao, Kai Ning Fang, Jing Wang, Yan Tang, Shohreh Shahabi, Xiao Yan Chen |
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| Rok vydání: | 2018 |
| Předmět: |
endocrine system diseases
mRNA overall survival HE4 02 engineering and technology Malignancy medicine.disease_cause Benign tumor 03 medical and health sciences 0302 clinical medicine Eukaryotic translation medicine Initiation factor Messenger RNA business.industry 021001 nanoscience & nanotechnology medicine.disease Epididymis female genital diseases and pregnancy complications epithelial ovarian cancer (EOC) medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research eIF3a Immunohistochemistry 0210 nano-technology business Carcinogenesis Research Paper |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| Popis: | Human epididymis protein 4 (HE4) is one of the most promising biomarkers for epithelial ovarian cancer (EOC). The majority of previous studies utilized the serum level or tissue protein expression of HE4 based upon immunohistochemistry (IHC) to evaluate the role of HE4 in the diagnosis, prognosis, and surveillance of EOC, but very little is known about HE4 mRNA expression. Eukaryotic translation initiation factor 3a (eIF3a) is implicated in oncogenesis and has been investigated extensively as a potential biomarker for malignancy. We previously reported a positive correlation between IHC expressions of eIF3a and HE4 in EOC. In the present study, we performed RT-PCR to determine mRNA expressions of HE4 and eIF3a in 30 normal ovarian tissues, 45 benign, 20 borderline and 94 malignant ovarian tumors. The association of HE4 and eIF3a mRNA expressions with clinicopathological characteristics and patient survivals was investigated. IHC was also performed in the same participants to investigate the correlation between mRNA and protein levels of HE4. HE4 mRNA level was found to be 48.42 ± 74.55 (mean ± SD, range: 0.01-343.99), significantly higher in primary EOC than in the borderline tumor, benign tumor, and normal ovarian tissue (P0.05). Collectively, our study revealed that increased HE4 mRNA expression correlates with high level of eIF3a mRNA and better survival in women with EOC, which calls for further investigations. |
| Databáze: | OpenAIRE |
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