Identification of a pathogenicity determinant of Plum pox virus in the sequence encoding the C-terminal region of protein P3 + 6K1
| Autor: | Silvie Dallot, Laurence Quiot, Pilar Sáenz, José Luis Riechmann, María Teresa Cervera, Juan Antonio García, Jean-Bernard Quiot |
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| Přispěvatelé: | Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), UMR INRA / ENSAM / CIRAD : Biologie et Génétique des Interactions Plantes / Parasite pour la Protection Intégrée, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Montpellier (ENSA M), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
0106 biological sciences
DNA Complementary Molecular Sequence Data Genome Viral Biology 01 natural sciences Genome Virus 03 medical and health sciences Viral Proteins Plasmid Virology Plant virus Complementary DNA Tobacco Coding region Amino Acid Sequence Cloning Molecular 030304 developmental biology DNA Primers Genetics [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology 0303 health sciences Base Sequence Sequence Homology Amino Acid Virulence Potyvirus Peas biology.organism_classification Plants Toxic Phenotype Capsid Genes Plum Pox Virus DNA Viral [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology identification 010606 plant biology & botany |
| Zdroj: | Journal of General Virology Journal of General Virology, Microbiology Society, 2000, 81, pp.557-566 Europe PubMed Central |
| ISSN: | 0022-1317 1465-2099 |
| Popis: | UMR BGPI Equipe 6 Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; A full-length genomic cDNA clone of a plum pox potyvirus (PPV) isolate belonging to the M strain (PPV-PS) has been cloned downstream from a bacteriophage T7 polymerase promoter and sequenced. Transcripts from the resulting plasmid, pGPPVPS, were infectious and, in herbaceous hosts, produced symptoms that differed from those of virus progeny of pGPPV, a full-length genomic cDNA clone of the D strain PPV-R. Viable PPV-R/-PS chimeric viruses were constructed by recombination of the cDNA clones in vitro. Analysis of plants infected with the different chimeras indicated that sequences encoding the most variable regions of the potyvirus genome, the P1 and capsid protein coding sequences, were not responsible for symptom differences between the two PPV isolates in herbaceous hosts. On the contrary, complex symptomatology determinants seem to be located in the central region of the PPV genome. The results indicate that a genomic fragment that encodes 173 aa from the C-terminal part of the P3+6K1 coding region is enough to confer, on a PPV-R background, a PS phenotype in Nicotiana clevelandii. This pathogenicity determinant also participates in symptom induction in Pisum sativum, although the region defining the PS phenotype in this host is probably restricted to 74 aa |
| Databáze: | OpenAIRE |
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