Cooperativity of sequence elements mediates tissue specificity of the rat insulin II gene
| Autor: | Ming-Jer Tsai, Yi-Zhong Gu, Young-Ping Hwung |
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| Rok vydání: | 1990 |
| Předmět: |
Ovalbumin
Mutagenesis (molecular biology technique) Cooperativity Biology Cell Line Ribonucleases Genes Regulator Gene expression Animals Insulin Promoter Regions Genetic Molecular Biology Gene Regulation of gene expression Nucleotide Mapping Promoter Cell Biology Transfection Molecular biology Rats Gene Expression Regulation Genes Cell culture Oligonucleotide Probes Chickens Research Article |
| Zdroj: | Molecular and Cellular Biology. 10:1784-1788 |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.10.4.1784 |
| Popis: | The 5'-flanking region of the rat insulin II gene (-448 to +50) is sufficient for tissue-specific expression. To further determine the tissue-specific cis-acting element(s), important sequences defined by linker-scanning mutagenesis were placed upstream of a heterologous promoter and transfected into insulin-producing and -nonproducing cells. Rat insulin promoter element 3 (RIPE3), which spans from -125 to -86, was shown to confer beta-cell-specific expression in either orientation. However, two subregions of RIPE3, RIPE3a and RIPE3b (defined by linker-scanning mutations), displayed only marginal activities. These results suggest that the two subregions cooperate to confer tissue specificity, presumably via their cognate binding factors. |
| Databáze: | OpenAIRE |
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