T cell maturation stage-linked heterogeneity of the glycosylphosphatidylinositol membrane anchor of Thy-1
| Autor: | I Nakashima, Mei Yi Pu, Tint Lwin, Hiroh Ikezawa, Ken-ichi Isobe, Takashi Iwamoto, Masashi Kato, Tomoaki Yoshida, Ryo Taguchi, Yue-Hua Zhang, Mahbuba Parvin |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Glycosylphosphatidylinositols
CD3 T cell T-Lymphocytes Immunology Hydroxylamine Hydroxylamines chemistry.chemical_compound Mice Phosphoinositide Phospholipase C Antigen Bacterial Proteins Phosphoinositide phospholipase C medicine Immunology and Allergy Animals Phosphatidylinositol Mice Inbred C3H Membrane Glycoproteins biology Phospholipase C Phosphoric Diester Hydrolases Phosphatidylinositol Diacylglycerol-Lyase Hematology Molecular biology In vitro Thymocyte medicine.anatomical_structure Biochemistry chemistry Antigens Surface biology.protein Thy-1 Antigens |
| Zdroj: | Immunobiology. 185(5) |
| ISSN: | 0171-2985 |
| Popis: | We showed that some of Thy-1 molecules on murine thymocytes are resistant to phosphatidylinositol-specific phospholipase C (PI-PLC) derived from Bacillus thuringiensis. Both immature thymocytes with low CD3 expression and mature thymic T lymphocytes with high CD3 expression carried the PI-PLC-resistant Thy-1, and the PI-PLC-sensitivity of Thy-1 extensively varied among thymocyte subpopulations. In contrast, the same PI-PLC fully hydrolysed the anchor of Thy-1 on peripheral T lymphocytes. When the latter cells were activated with mitogen in vitro, however, some Thy-1 on them became resistant to PI-PLC. We then found that virtually all Thy-1 molecules on thymocytes became sensitive to PI-PLC when they were treated with hydroxylamine that should cleave ester-linked lipids. The result ruled out the possibility that the PI-PLC-resistant Thy-1 had a transmembranous peptide sequence, and suggested the presence of an additional fatty acyl group on the inositol ring of the Thy-1 anchor. In addition, the molecular size of the PI-PLC-resistant membrane-bound Thy-1 was only marginally larger than that of the PI-PLC-sensitive solubilized Thy-1 in detergent-partitioning SDS-PAGE analysis. |
| Databáze: | OpenAIRE |
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