Centriole and Golgi microtubule nucleation are dispensable for the migration of human neutrophil-like cells
| Autor: | Laurel E. Hind, Lucas C. Klemm, Ryan A. Denu, Anna Huttenlocher, Briana L. Rocha-Gregg, Mark E. Burkard |
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| Rok vydání: | 2021 |
| Předmět: |
Centriole
Neutrophils Golgi Apparatus Motility Biology Microtubules Cell Line symbols.namesake Cell Movement Microtubule Animals Humans Cytoskeleton Molecular Biology Centrioles Microtubule nucleation Microtubule organizing center Chemotaxis Cell Biology Golgi apparatus Cell biology HEK293 Cells symbols Brief Reports Microtubule-Organizing Center |
| Zdroj: | Molecular Biology of the Cell |
| ISSN: | 1939-4586 1059-1524 |
| Popis: | Neutrophils migrate in response to chemoattractants to mediate host defense. Chemoattractants drive rapid intracellular cytoskeletal rearrangements including the radiation of microtubules from the microtubule-organizing center (MTOC) towards the rear of polarized neutrophils. Microtubules regulate neutrophil polarity and motility, but little is known about the specific role of MTOCs. To characterize the role of MTOCs on neutrophil motility we depleted centrioles in a well-established neutrophil-like cell line. Surprisingly, both chemical and genetic centriole depletion increased neutrophil speed and chemotactic motility, suggesting an inhibitory role for centrioles during directed migration. We also found that depletion of both centrioles and GM130-mediated Golgi microtubule nucleation did not impair neutrophil directed migration. Taken together, our findings demonstrate an inhibitory role for centrioles and a resilient MTOC system in motile human neutrophil-like cells. [Media: see text] [Media: see text] [Media: see text]. |
| Databáze: | OpenAIRE |
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