Molecular analysis of the 2012 Bundibugyo virus disease outbreak
| Autor: | Joseph N. Fair, Maria Makuwa, Jens H. Kuhn, Gustavo Palacios, Jean-Jacques Muyembe-Tamfum, Jeffrey R. Kugelman, Prime Mulembakani, Raina Kumar, Joshua Richardson, Nicholas Di Paola, Elyse R. Nagle, Randal J. Schoepp, Nadia Wauquier, Jarod Hanson, Christine E. Hulseberg, Mariano Sanchez-Lockhart, Peter A. Larson |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent EBOD Genome Viral Bundibugyo virus disease Disease medicine.disease_cause Polymorphism Single Nucleotide molecular epidemiology General Biochemistry Genetics and Molecular Biology Disease Outbreaks Bundibugyo virus Ebola disease Report Chlorocebus aethiops Epidemiology medicine Animals Humans Vero Cells Pathogen Phylogeny Aged BVD biology Molecular epidemiology Outbreak Bayes Theorem Hemorrhagic Fever Ebola Middle Aged Ebolavirus biology.organism_classification Virology BDBV Bundibugyo ebolavirus Molecular analysis Haplotypes Child Preschool Ebola Female |
| Zdroj: | Cell Reports Medicine |
| ISSN: | 2666-3791 |
| DOI: | 10.1016/j.xcrm.2021.100351 |
| Popis: | Summary Bundibugyo virus (BDBV) is one of four ebolaviruses known to cause disease in humans. Bundibugyo virus disease (BVD) outbreaks occurred in 2007–2008 in Bundibugyo District, Uganda, and in 2012 in Isiro, Province Orientale, Democratic Republic of the Congo. The 2012 BVD outbreak resulted in 38 laboratory-confirmed cases of human infection, 13 of whom died. However, only 4 BDBV specimens from the 2012 outbreak have been sequenced. Here, we provide BDBV sequences from seven additional patients. Analysis of the molecular epidemiology and evolutionary dynamics of the 2012 outbreak with these additional isolates challenges the current hypothesis that the outbreak was the result of a single spillover event. In addition, one patient record indicates that BDBV’s initial emergence in Isiro occurred 50 days earlier than previously accepted. Collectively, this work demonstrates how retrospective sequencing can be used to elucidate outbreak origins and provide epidemiological contexts to a medically relevant pathogen. Graphical abstract Highlights In 2012, BDBV was circulating earlier than previously appreciated BDBV genomes from seven additional patients are sequenced Molecular analyses indicate that multiple spillover events fueled the BVD outbreak Elucidation of the epidemiology underlying the 2012 Bundibugyo virus disease outbreak in the Democratic Republic of the Congo has been challenging. Hulseberg et al. acquire additional genomic and phylodynamic data indicating that multiple Bundibugyo virus spillover events, some of which occurred much earlier than previously known, contributed to the outbreak. |
| Databáze: | OpenAIRE |
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