In vivo performance of freeze-dried decellularized pulmonary heart valve allo- and xenografts orthotopically implanted into juvenile sheep
| Autor: | Klaus Höffler, Serghei Cebotari, Igor Tudorache, Andrés Vásquez Rivera, Robert Ramm, Willem F. Wolkers, Axel Haverich, Andres Hilfiker, Anatol Ciubotaru, Samir Sarikouch, Tobias Goecke, Karolina Theodoridis |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Protein Denaturation Transplantation Heterologous Biomedical Engineering Hemodynamics Fluorescent Antibody Technique 030204 cardiovascular system & hematology Matrix (biology) Biochemistry Biomaterials 03 medical and health sciences 0302 clinical medicine In vivo medicine Animals Transplantation Homologous Heart valve Molecular Biology Sodium dodecylsulfate Pulmonary Valve Decellularization Sheep Calorimetry Differential Scanning business.industry Temperature General Medicine Extracellular Matrix 030104 developmental biology medicine.anatomical_structure Freeze Drying Heart Valve Prosthesis Female business Limited resources Biotechnology Large animal |
| Zdroj: | Acta biomaterialia. 68 |
| ISSN: | 1878-7568 |
| Popis: | The decellularization of biological tissues decreases immunogenicity, allows repopulation with cells, and may lead to improved long-term performance after implantation. Freeze drying these tissues would ensure off-the-shelf availability, save storage costs, and facilitates easy transport. This study evaluates the in vivo performance of freeze-dried decellularized heart valves in juvenile sheep. TritonX-100 and sodium dodecylsulfate decellularized ovine and porcine pulmonary valves (PV) were freeze-dried in a lyoprotectant sucrose solution. After rehydration for 24 h, valves were implanted into the PV position in sheep as allografts (fdOPV) and xenografts (fdPPV), while fresh dezellularized ovine grafts (frOPV) were implanted as controls. Functional assessment was performed by transesophageal echocardiography at implantation and at explantation six months later. Explanted grafts were analysed histologically to assess the matrix, and immunofluorescence stains were used to identify the repopulating cells. Although the graft diameters and orifice areas increased, good function was maintained, except for one insufficient, strongly deteriorated frOPV. Cells which were positive for either endothelial or interstitial markers were found in all grafts. In fdPPV, immune-reactive cells were also found. Our findings suggest that freeze-drying does not alter the early hemodynamic performance and repopulation potential of decellularized grafts in vivo, even in the challenging xenogeneic situation. Despite evidence of an immunological reaction for the xenogenic valves, good early functionalities were achieved. Statement of Significance Decellularized allogeneic heart valves show excellent results as evident from large animal experiments and clinical trials. However, a long-term storing method is needed for an optimal use of this limited resource in the clinical setting, where an optimized matching of graft and recipient is requested. As demonstrated in this study, freeze-dried and freshly decellularized grafts reveal equally good results after implantation in the juvenile sheep concerning function and repopulation with recipients’ cells. Thus, freeze-drying arises as a promising method to extend the shelf-life of valvular grafts compared to those stored in antibiotic-solution as currently practised. |
| Databáze: | OpenAIRE |
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